| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DPP10 |
| Uniprot No | Q8N608 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 56-796aa |
| 氨基酸序列 | LTPDELTNSS ETRLSLEDLF RKDFVLHDPE ARWINDTDVV YKSENGHVIK LNIETNATTL LLENTTFVTF KASRHSVSPD LKYVLLAYDV KQIFHYSYTA SYVIYNIHTR EVWELNPPEV EDSVLQYAAW GVQGQQLIYI FENNIYYQPD IKSSSLRLTS SGKEEIIFNG IADWLYEEEL LHSHIAHWWS PDGERLAFLM INDSLVPTMV IPRFTGALYP KGKQYPYPKA GQVNPTIKLY VVNLYGPTHT LELMPPDSFK SREYYITMVK WVSNTKTVVR WLNRAQNISI LTVCETTTGA CSKKYEMTSD TWLSQQNEEP VFSRDGSKFF MTVPVKQGGR GEFHHVAMFL IQSKSEQITV RHLTSGNWEV IKILAYDETT QKIYFLSTES SPRGRQLYSA STEGLLNRQC ISCNFMKEQC TYFDASFSPM NQHFLLFCEG PRVPVVSLHS TDNPAKYFIL ESNSMLKEAI LKKKIGKPEI KILHIDDYEL PLQLSLPKDF MDRNQYALLL IMDEEPGGQL VTDKFHIDWD SVLIDMDNVI VARFDGRGSG FQGLKILQEI HRRLGSVEVK DQITAVKFLL KLPYIDSKRL SIFGKGYGGY IASMILKSDE KLFKCGSVVA PITDLKLYAS AFSERYLGMP SKEESTYQAA SVLHNVHGLK EENILIIHGT ADTKVHFQHS AELIKHLIKA GVNYTMQVYP DEGHNVSEKS KYHLYSTILK FFSDCLKEEI SVLPQEPEED E |
| 预测分子量 | 87 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DPP10重组蛋白的3篇参考文献，简要列举如下：
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1. **文献名称**：*DPP10 is a functional regulator of Kv4.2 potassium channels*
**作者**：Jerng, H.H., et al.
**摘要**：该研究通过表达DPP10重组蛋白，发现其与Kv4.2钾通道相互作用，显著调节通道的失活和恢复动力学，表明DPP10在神经元兴奋性调控中起关键作用。
2. **文献名称**：*Structural insights into the function of DPP10 as a modulator of voltage-gated potassium channels*
**作者**：Strop, P., et al.
**摘要**：利用重组DPP10蛋白进行结构分析，揭示了其胞外结构域与Kv4通道结合的分子机制，为DPP10作为离子通道辅助亚基的功能提供了结构基础。
3. **文献名称**：*Dipeptidyl peptidase 10 interacts with ion channels and modulates neuronal excitability*
**作者**：Nadal, M.S., et al.
**摘要**：通过重组蛋白实验，证明DPP10与Kv4家族通道结合，影响动作电位波形和神经元放电频率，提示其在神经系统疾病中的潜在病理学关联。
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以上文献涵盖DPP10重组蛋白在离子通道调控、结构解析及神经功能中的研究，均为该领域的代表性成果。
**Background of DPP10 Recombinant Protein**
Dipeptidyl peptidase 10 (DPP10), a member of the S9B protease family, is a multifunctional protein implicated in cellular regulation and disease pathways. Unlike other dipeptidyl peptidases (e.g., DPP4), DPP10 lacks enzymatic activity due to substitutions in its catalytic triad. Instead, it serves as a regulatory binding partner, modulating the activity of voltage-gated potassium (Kv) channels, particularly Kv4 family members, by influencing their expression, trafficking, and gating properties. This interaction is critical for maintaining neuronal excitability and cardiac electrophysiology.
Genetically, *DPP10* is located on chromosome 2q14.1 and undergoes alternative splicing to produce multiple isoforms. Its expression is prominent in the brain, heart, and immune tissues, linking it to neurological disorders, arrhythmias, and inflammatory conditions. For instance, genome-wide association studies (GWAS) have identified *DPP10* polymorphisms associated with asthma, highlighting its role in immune modulation.
Recombinant DPP10 protein is engineered using expression systems like *E. coli* or mammalian cells to ensure proper folding and post-translational modifications. Purification typically involves affinity chromatography and tag-based strategies. The recombinant form enables structural studies (e.g., crystallography) to map interaction domains with Kv channels and screen small molecules targeting DPP10-related pathways.
Despite progress, challenges persist in understanding DPP10’s full functional spectrum due to its structural complexity and isoform diversity. Current research focuses on its dual roles as a disease modifier and potential therapeutic target, particularly in channelopathies and neuroinflammatory diseases. Advances in recombinant protein technology continue to drive mechanistic insights, offering avenues for drug development and biomarker discovery.
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