关键信息
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基因名
GMDS
- 应用
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别名
GMDS;GDP-mannose 4.6 dehydratase
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O60547
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表达区间
1-372aa
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氨基酸序列
MGSSHHHHHHSSGLVPRGSHMAHAPARCPSARGSGDGEMGKPRNVALITG ITGQDGSYLAEFLLEKGY EVHGIVRRSSSFNTGRIEHLYKNPQAHIEG NMKLHYGDLTDSTCLVKIINEVKPTEIYNLGAQSHVKISFDLAEY TAD VDGVGTLRLLDAVKTCGLINSVKFYQASTSELYGKVQEIPQKETTPFYPR SPYGAAKLYAYWIVVNFREAYN LFAVNGILFNHESPRRGANFVTRKIS RSVAKIYLGQLECFSLGNLDAKRDWGHAKDYVEAMWLMLQNDEPEDFVI ATGEVHSVREFVEKSFLHIGKTIVWEGKNENEVGRCKETGKVHVTVDLK YYRPTEVDFLQGDCTKAKQKLNWKPR VAFDELVREMVHADVELMRTNP NA
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分子量
44 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
GMDS (GDP-mannose 4,6-dehydratase) is an enzyme that plays a critical role in the biosynthesis of GDP-mannose, a precursor for various glycosylation processes essential for proper cellular function. Research into GMDS has gained traction due to its potential implications in human health and disease. Alterations in glycosylation pathways have been associated with a range of conditions, including congenital disorders, cancer, and infectious diseases. Understanding the structure and function of GMDS can provide insights into these pathways and their dysregulation. Additionally, the recombinant production of GMDS offers a valuable tool for studying its enzymatic mechanisms and interactions with other cellular components. By utilizing techniques such as molecular cloning and protein expression systems, researchers can produce GMDS in significant quantities, facilitating enzymatic assays and structural studies. Moreover, GMDS as a potential therapeutic target highlights the significance of continued research in this area. Investigating the enzyme’s regulation, substrate specificity, and interaction with other glycosylation-related enzymes could reveal novel strategies for therapeutic interventions. Overall, the ongoing research on GMDS recombinant protein not only enhances our understanding of glycosylation but may also lead to innovative approaches for treating various diseases resulting from glycosylation defects.
