| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Death-associated protein kinase 1, DAP kinase 1, 2.7.11.1, DAPK1, DAPK |
| Entrez GeneID | 1612 |
| WB Predicted band size | 160.0kDa |
| Host/Isotype | Mouse IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between amino acids from human. |
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以下是关于hDAPK1-T1316抗体的虚构参考文献示例(仅供示例用途)：
1. **文献名称**：*Phosphorylation of DAPK1 at T1316 modulates its pro-apoptotic function in breast cancer cells*
**作者**：Kim J. et al.
**摘要**：本研究利用hDAPK1-T1316特异性抗体，揭示了DAPK1在T1316位点的磷酸化通过抑制其激酶活性，降低促凋亡功能，从而促进乳腺癌细胞存活。该发现为靶向DAPK1磷酸化的癌症治疗提供了新思路。
2. **文献名称**：*Development and validation of a novel hDAPK1-T1316 phospho-specific antibody for neurodegenerative disease research*
**作者**：Chen L. et al.
**摘要**：作者开发并验证了hDAPK1-T1316磷酸化特异性抗体，证明其在阿尔茨海默病模型中可特异性检测DAPK1的磷酸化状态。研究显示，T1316磷酸化水平升高与tau蛋白异常磷酸化及神经元死亡相关。
3. **文献名称**：*T1316 phosphorylation of DAPK1 promotes autophagy in hypoxia-induced cardiac injury*
**作者**：Wang X. et al.
**摘要**：通过hDAPK1-T1316抗体，本研究证实缺氧条件下DAPK1的T1316位点磷酸化增强，进而激活自噬通路，加剧心肌细胞损伤。该机制为心脏缺血再灌注损伤的干预提供了潜在靶点。
4. **文献名称**：*Epigenetic regulation of DAPK1-T1316 phosphorylation in colorectal cancer metastasis*
**作者**：Garcia R. et al.
**摘要**：研究利用hDAPK1-T1316抗体发现，DNA甲基化异常导致DAPK1 T1316磷酸化水平降低，促进结直肠癌转移。该抗体被用于临床样本分析，证实其作为预后标志物的潜力。
(注：以上内容为模拟生成，实际文献需通过学术数据库检索。)
The hDAPK1-T1316 antibody is a phospho-specific antibody designed to detect human Death-associated protein kinase 1 (DAPK1) phosphorylated at threonine residue 1316 (T1316). DAPK1 is a calcium/calmodulin-regulated serine/threonine kinase involved in diverse cellular processes, including apoptosis, autophagy, and membrane blebbing. Its activity is tightly regulated by phosphorylation events, with autophosphorylation at T1316 being a critical modification that stabilizes the inactive state of the kinase by promoting its interaction with calmodulin. The T1316 phosphorylation site is located within the autophagic signaling domain, linking DAPK1 to pathways regulating cell survival and death.
This antibody is particularly useful in studying the regulatory mechanisms of DAPK1 activation under physiological or pathological conditions, such as cancer, neurodegenerative diseases, or ischemic injury. By specifically recognizing phosphorylated T1316. it enables researchers to assess the inactive conformation of DAPK1 in various experimental models, including Western blotting, immunohistochemistry, and immunofluorescence. Validated for specificity and sensitivity, the hDAPK1-T1316 antibody serves as a key tool for investigating DAPK1's role in cellular stress responses and its dysregulation in disease contexts. Its applications extend to drug discovery studies targeting DAPK1-mediated pathways for therapeutic intervention.
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