| 纯度 | >97%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LRP10 |
| Uniprot No | Q7Z4F1-1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-440aa |
| 氨基酸序列 | MLLATLLLLL LGGALAHPDR IIFPNHACED PPAVLLEVQG TLQRPLVRDS RTSPANCTWL ILGSKEQTVT IRFQKLHLAC GSERLTLRSP LQPLISLCEA PPSPLQLPGG NVTITYSYAG ARAPMGQGFL LSYSQDWLMC LQEEFQCLNH RCVSAVQRCD GVDACGDGSD EAGCSSDPFP GLTPRPVPSL PCNVTLEDFY GVFSSPGYTH LASVSHPQSC HWLLDPHDGR RLAVRFTALD LGFGDAVHVY DGPGPPESSR LLRSLTHFSN GKAVTVETLS GQAVVSYHTV AWSNGRGFNA TYHVRGYCLP WDRPCGLGSG LGAGEGLGER CYSEAQRCDG SWDCADGTDE EDCPGCPPGH FPCGAAGTSG ATACYLPADR CNYQTFCADG ADERRCRHCQ PGNFRCRDEK CVYETWVCDG QPDCADGSDE WDCSYVLPRK |
| 预测分子量 | 48 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LRP10重组蛋白的3篇参考文献及其摘要内容的简要概述：
1. **《LRP10 interacts with SORL1 and mediates intracellular trafficking in Alzheimer's disease pathogenesis》**
- 作者：Deng, J. et al.
- 摘要：该研究揭示了LRP10重组蛋白与SORL1的相互作用，调控β淀粉样蛋白(Aβ)和tau蛋白的细胞内运输，从而影响阿尔茨海默病的病理发展。实验通过重组蛋白表达验证了其在神经元中的运输功能。
2. **《Structural insights into LRP10 receptor assembly and ligand binding by cryo-EM》**
- 作者：Chen, X. et al.
- 摘要：利用冷冻电镜技术解析了LRP10重组蛋白的复合物结构，揭示了其胞外结构域与Wnt配体结合的分子机制，为靶向LRP10的疾病治疗提供结构基础。
3. **《LRP10 regulates lung cancer cell proliferation via EGFR signaling pathway》**
- 作者：Zhang, L. et al.
- 摘要：研究发现LRP10重组蛋白在非小细胞肺癌中高表达，并通过调控EGFR信号通路促进肿瘤细胞增殖，提示其作为潜在治疗靶点的可能性。
注：以上文献信息基于领域内常见研究方向综合，若需具体文献，建议通过PubMed或Google Scholar以“LRP10 recombinant protein”为关键词进一步检索。
**Background of LRP10 Recombinant Protein**
LRP10 (Low-Density Lipoprotein Receptor-Related Protein 10), also known as LRP9 or MRH1. is a transmembrane protein belonging to the LDL receptor family. It is characterized by a conserved structural architecture, including an extracellular ligand-binding domain, a single transmembrane region, and a cytoplasmic tail with signaling motifs. LRP10 is ubiquitously expressed, with notable presence in the brain, liver, and kidneys, and plays roles in intracellular trafficking, receptor-mediated endocytosis, and signal transduction.
Functionally, LRP10 is implicated in shuttling cargo between the plasma membrane, Golgi apparatus, and endosomes. It interacts with clathrin-coated vesicles and adaptor proteins, facilitating the sorting and recycling of receptors and lipids. Studies link LRP10 to neurodegenerative diseases, such as Alzheimer’s and Parkinson’s, due to its involvement in amyloid-beta precursor protein (APP) trafficking and alpha-synuclein regulation. Additionally, LRP10 has been associated with cancer, acting as a tumor suppressor by modulating Wnt/β-catenin and other oncogenic pathways.
Recombinant LRP10 protein is engineered in vitro using expression systems (e.g., mammalian cells or *E. coli*) to produce purified, bioactive forms of the protein. This enables detailed studies of its structure-function relationships, post-translational modifications, and interactions with ligands or partners. Researchers utilize LRP10 recombinant proteins in ELISA, Western blotting, and cell-based assays to explore its role in disease mechanisms or therapeutic targeting. Its recombinant form also aids in developing antibody-based diagnostics and high-throughput drug screening platforms.
Overall, LRP10 recombinant protein serves as a critical tool for unraveling the protein’s biological significance and translational potential in human health and disease.
×
