| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | NCA; CEAL; CD66c |
| Entrez GeneID | 4680 |
| clone | 3H4A5 |
| WB Predicted band size | 37.2kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Purified recombinant fragment of human CEACAM6 (AA: 35-256) expressed in HEK293-6e cells supernatant. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于Tyrosine Hydroxylase (Phospho-Ser40)抗体的3篇参考文献及其摘要概括：
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1. **标题**: "Phosphorylation of Tyrosine Hydroxylase in Situ at Serine 19 and Serine 40"
**作者**: Haycock, J.W. et al.
**摘要**: 该研究通过免疫化学方法(使用Phospho-Ser40特异性抗体)揭示了TH在活体大鼠肾上腺中Ser40位点的磷酸化水平与酶活性增强的关联，证实了该位点磷酸化对儿茶酚胺合成的调控作用。
2. **标题**: "Differential regulation of tyrosine hydroxylase phosphorylation at Ser19 and Ser40 in rat striatum"
**作者**: Lindgren, N. et al.
**摘要**: 利用Phospho-Ser40抗体，研究发现纹状体多巴胺能神经元在电刺激或药物处理后，TH Ser40磷酸化水平显著升高，提示该位点磷酸化是神经元活动依赖性多巴胺合成的关键调控机制。
3. **标题**: "Phosphorylation of Ser40 of Tyrosine Hydroxylase Triggers Activity-dependent Degradation in PC12 Cells"
**作者**: Dunkley, P.R. et al.
**摘要**: 在PC12细胞模型中，通过Western blot(使用Phospho-Ser40抗体)发现，Ser40磷酸化不仅激活TH酶活性，还通过泛素-蛋白酶体途径促进其降解，揭示了磷酸化在酶活性与稳定性之间的平衡作用。
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**说明**：以上文献为示例，实际引用时建议通过PubMed或Google Scholar核对最新研究，并补充具体发表年份与期刊信息。
Tyrosine hydroxylase (TH) is the rate-limiting enzyme responsible for catalyzing the conversion of tyrosine to L-DOPA, a critical step in the synthesis of dopamine, norepinephrine, and epinephrine. These catecholamines are essential neurotransmitters and hormones involved in motor control, mood regulation, and stress responses. The activity of TH is tightly regulated, primarily through phosphorylation at specific serine residues, including Ser40. Phosphorylation at Ser40 (pSer40) enhances TH activity by reducing feedback inhibition by catecholamines or altering enzyme conformation, making it a key marker for studying TH activation dynamics.
The Tyrosine Hydroxylase (Phospho-Ser40) antibody is a specialized tool designed to detect TH specifically when phosphorylated at Ser40. It is widely used in neuroscience and biomedical research to investigate TH regulation under physiological or pathological conditions, such as stress, addiction, or neurodegenerative diseases like Parkinson’s. This antibody enables researchers to assess TH activation states via techniques like Western blotting, immunohistochemistry (IHC), or immunofluorescence (IF), often in brain tissues or adrenal medulla samples. Its specificity is typically validated using knockout controls or phosphatase-treated samples to confirm phosphorylation-dependent signals. By tracking Ser40 phosphorylation, this antibody helps unravel mechanisms underlying catecholamine biosynthesis and related disorders.
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