Rabbit Polyclonal EGFR(Phospho-Tyr1197) Antibody

The EGFR (Phospho-Tyr1197) antibody specifically detects epidermal growth factor receptor (EGFR) when phosphorylated at tyrosine residue 1197 (Y1197), a key post-translational modification associated with receptor activation. EGFR, a transmembrane tyrosine kinase receptor, plays a critical role in regulating cell proliferation, survival, and differentiation. Upon ligand binding (e.g., EGF, TGF-α), EGFR undergoes dimerization and autophosphorylation at specific tyrosine residues within its intracellular kinase domain, including Y1197. This phosphorylation event facilitates downstream signaling by recruiting adaptor proteins (e.g., GRB2. SHC) to activate pathways such as MAPK/ERK, PI3K/AKT, and JAK/STAT, which drive cellular responses. Dysregulated EGFR signaling, often linked to hyperphosphorylation or mutations, is implicated in cancer progression, metastasis, and therapeutic resistance. The EGFR (Phospho-Tyr1197) antibody is widely used in research to study EGFR activation status, particularly in cancer models, drug development, and mechanistic studies of receptor trafficking and degradation. It is validated for applications like Western blotting, immunohistochemistry, and immunofluorescence, helping to elucidate EGFR’s role in both physiological and pathological contexts. Detection of Y1197 phosphorylation may also serve as a biomarker for targeted therapy efficacy or resistance in EGFR-driven malignancies.