Rabbit Polyclonal GSK3α/β(Phospho-Tyr279/216) Antibody

The GSK3α/β (Phospho-Tyr279/216) antibody is a specialized tool used to detect the phosphorylated forms of glycogen synthase kinase 3 alpha (GSK3α) and beta (GSK3β) at tyrosine residues 279 (α) and 216 (β). GSK3 is a serine/threonine kinase involved in critical cellular processes, including glucose metabolism, Wnt/β-catenin signaling, and apoptosis. Unlike its inactivation via serine phosphorylation (e.g., Ser21 in GSK3α or Ser9 in GSK3β), tyrosine phosphorylation at these sites (Tyr279/216) is associated with basal kinase activity regulation. Studies suggest that Tyr216 phosphorylation in GSK3β enhances its kinase activity, though its precise role in GSK3α remains less characterized. This antibody is widely utilized in research to explore GSK3 activation states in diseases such as diabetes, neurodegenerative disorders (e.g., Alzheimer’s), and cancer, where dysregulated GSK3 signaling contributes to pathogenesis. It enables detection of phosphorylated GSK3α/β in techniques like Western blotting, immunohistochemistry, or immunofluorescence, aiding investigations into cellular signaling dynamics, drug response, and therapeutic targeting. Researchers often pair it with total GSK3 or inhibition-specific antibodies to contextualize activation-inhibition balance in experimental models.