| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PRMT4 |
| Entrez GeneID | 10498; |
| WB Predicted band size | 63kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of serine 228(V-K-S(p)-N-N) derived from Human CARM1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于CARM1(Phospho-Ser228)抗体的3篇假设性参考文献示例，基于典型研究场景构建：
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1. **文献名称**：*Phosphorylation of CARM1 at Ser228 Modulates Its Methyltransferase Activity and Chromatin Recruitment*
**作者**：Zhang et al.
**摘要**：本研究揭示了CARM1在Ser228位点的磷酸化对其精氨酸甲基转移酶活性的调控作用。通过质谱分析和体外激酶实验，作者发现CDK1介导的Ser228磷酸化增强了CARM1与组蛋白H3的结合能力，从而促进靶基因的转录激活。实验中使用的CARM1(Phospho-Ser228)抗体被验证具有高度特异性，可用于免疫印迹和免疫荧光检测。
2. **文献名称**：*A Kinase-Driven Phosphorylation Switch on CARM1 Regulates Estrogen Receptor Signaling*
**作者**：Lee et al.
**摘要**：该研究报道了DNA损伤信号通过ATM激酶诱导CARM1 Ser228磷酸化，进而调控雌激素受体(ERα)的转录共激活功能。利用CARM1(Phospho-Ser228)抗体的ChIP-seq分析显示，磷酸化的CARM1优先富集于ERα靶基因启动子区域，促进乳腺癌细胞增殖。
3. **文献名称**：*Development and Validation of a Phospho-Specific Antibody for CARM1 Ser228 Phosphorylation Studies*
**作者**：Brown et al.
**摘要**：本文详细描述了针对CARM1 Ser228磷酸化位点的多克隆抗体的开发流程，包括抗原肽设计、动物免疫和抗体特异性验证(如磷酸化肽段竞争性抑制实验)。该抗体成功应用于检测细胞周期依赖性CARM1磷酸化动态变化，为相关功能研究提供了可靠工具。
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**注意**：以上文献为示例性内容，实际研究中请通过PubMed或Sci-Hub等平台检索真实发表的论文。若需具体文献，可进一步提供关键词或研究背景定向筛选。
The CARM1 (Phospho-Ser228) antibody is designed to detect the phosphorylation status of coactivator-associated arginine methyltransferase 1 (CARM1) at serine residue 228. CARM1. also known as PRMT4. is a member of the protein arginine methyltransferase family and plays critical roles in transcriptional regulation, chromatin remodeling, and cellular signaling by methylating histone H3 (Arg17. Arg26) and non-histone substrates. Phosphorylation at Ser228 is a post-translational modification that modulates CARM1’s enzymatic activity, subcellular localization, or interactions with other proteins. Studies suggest this modification may influence its role in processes like cell differentiation, apoptosis, and hormone-dependent gene activation.
The CARM1 (Phospho-Ser228) antibody is widely used in research to investigate the regulatory mechanisms of CARM1 in physiological and pathological contexts, such as cancer, inflammation, and metabolic disorders. It is validated for applications including Western blotting, immunoprecipitation, and immunofluorescence, enabling the study of CARM1 activation dynamics in response to signaling pathways (e.g., MAPK/ERK). Specificity is typically confirmed using phosphorylation-deficient mutants or peptide competition assays. This antibody serves as a key tool for elucidating how CARM1’s function is fine-tuned by phosphorylation, offering insights into its therapeutic potential in disease.
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