| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | TY3H; TYH; Tyrosine 3-hydroxylase; tyrosine hydroxylase; |
| Entrez GeneID | 7054; |
| WB Predicted band size | 55-60 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | Peptide sequence around phosphorylation site of serine19 (A-V-S(p)-E-Q) derived from Rat Tyrosine Hydroxylase (TH). |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Tyrosine Hydroxylase (Phospho-Ser19)抗体的3篇示例文献摘要(虚构内容，仅供示例参考)：
1. **文献名称**: *Phosphorylation of Tyrosine Hydroxylase at Ser19 modulates catecholamine synthesis in dopaminergic neurons*
**作者**: Smith A, et al.
**摘要**: 本研究利用Phospho-Ser19特异性抗体，揭示了在应激状态下，多巴胺能神经元中TH的Ser19磷酸化水平显著升高，并通过激活PKA通路促进儿茶酚胺的生物合成。
2. **文献名称**: *A novel role of TH phosphorylation at Ser19 in Parkinson's disease models*
**作者**: Chen L, et al.
**摘要**: 通过免疫印迹和免疫组化(使用Phospho-Ser19抗体)，发现帕金森病小鼠模型中TH的Ser19磷酸化异常降低，提示其可能与多巴胺能神经元退行性变相关。
3. **文献名称**: *Calcium-dependent regulation of Tyrosine Hydroxylase activity via Ser19 phosphorylation*
**作者**: Tanaka K, et al.
**摘要**: 研究结合Phospho-Ser19抗体和激酶抑制剂，证明CaMKII通过磷酸化TH的Ser19位点增强其酶活性，揭示了钙信号对儿茶酚胺动态平衡的调控机制。
(注：以上文献为模拟示例，实际引用需查询具体数据库如PubMed并核实信息。)
Tyrosine hydroxylase (TH) is the rate-limiting enzyme responsible for catalyzing the conversion of tyrosine to L-DOPA, a critical step in catecholamine biosynthesis (e.g., dopamine, norepinephrine). Its activity is tightly regulated by phosphorylation at specific serine residues, including Ser19. Ser31. and Ser40. The phosphorylation at Ser19. though less studied than Ser40. has been implicated in modulating TH activity, stability, or interactions with regulatory proteins. Antibodies targeting phospho-Ser19 (pSer19) of TH are essential tools for studying its post-translational modification and functional regulation under physiological or pathological conditions.
These antibodies are widely used in techniques like Western blotting, immunohistochemistry, or immunofluorescence to assess TH activation states in dopaminergic neurons or adrenal chromaffin cells. Research involving pSer19-specific antibodies has explored its role in stress responses, neurotransmitter synthesis, and diseases such as Parkinson’s disease, schizophrenia, or hypertension. Phosphorylation at Ser19 may be mediated by kinases like Ca²⁺/calmodulin-dependent protein kinase II (CaMKII) or protein kinase A (PKA), depending on cellular context. However, its exact regulatory mechanism remains less clear compared to other phosphorylation sites, warranting further investigation. Reliable pSer19 antibodies enable researchers to dissect TH’s complex regulation and its implications in neurobiology and disease.
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