| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Entrez GeneID | 891; |
| WB Predicted band size | 60kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Peptide sequence around phosphorylation site of Serine 147 (A-F-S(p)-D-V) derived from Human Cyclin B1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于 Cyclin B1 (phospho-Ser147) 抗体的3篇参考文献的简要信息：
1. **"DNA damage triggers Cyclin B1 phosphorylation via Plk1 to promote mitotic entry"**
- **作者**: Hirota, T. et al.
- **摘要**: 研究揭示了DNA损伤后，Plk1激酶磷酸化Cyclin B1的Ser147位点，促进其核转位和有丝分裂进入，使用phospho-Ser147抗体验证了该位点的修饰对细胞周期检查点的调控作用。
2. **"Phosphorylation of Cyclin B1 at Ser147 modulates cellular responses to microtubule stress"**
- **作者**: Burgess, A. et al.
- **摘要**: 通过免疫印迹和免疫荧光实验，发现微管损伤诱导Cyclin B1 Ser147磷酸化，该修饰依赖Aurora A激酶，并影响Cyclin B1/CDK1复合物的活性及细胞凋亡通路。
3. **"Cyclin B1 phosphorylation regulates DNA damage-induced G2/M arrest"**
- **作者**: Smits, V.A.J. & Medema, R.H.
- **摘要**: 利用phospho-Ser147特异性抗体，证明DNA损伤后ATR激酶介导的Cyclin B1磷酸化阻断了其与CDK1的结合，导致G2/M期阻滞，揭示了该位点在损伤修复中的关键作用。
*注：若需具体文献，建议通过PubMed或抗体供应商(如CST产品#4138)的引用列表获取全文细节。*
Cyclin B1 (phospho-Ser147) antibodies are essential tools for studying cell cycle regulation, particularly during the G2/M transition. Cyclin B1. a regulatory subunit of the cyclin-dependent kinase CDK1. forms a complex that drives mitotic entry by phosphorylating substrates critical for nuclear envelope breakdown and spindle assembly. Its activity is tightly controlled by subcellular localization and phosphorylation. Phosphorylation at Ser147. located in the cytoplasmic retention signal (CRS) domain, promotes Cyclin B1 nuclear translocation by disrupting interactions with exportin proteins. This post-translational modification typically occurs in late G2 phase, mediated by kinases such as CDK1 itself or Aurora A, ensuring timely mitotic progression.
Antibodies specific to Cyclin B1 phosphorylated at Ser147 enable researchers to detect and quantify this activated form in techniques like Western blotting, immunofluorescence, and immunohistochemistry. They are particularly valuable for investigating cell cycle checkpoints, DNA damage responses, and mitotic defects. In cancer research, these antibodies help assess dysregulated Cyclin B1 phosphorylation linked to uncontrolled proliferation or chemotherapy resistance. Proper validation using phosphorylation-deficient mutants or phosphatase-treated samples is crucial to confirm antibody specificity. Understanding Ser147 phosphorylation dynamics provides insights into cell cycle-related pathologies and therapeutic targeting of mitotic regulators.
×
