Rabbit Polyclonal ABL1/2(phospho-Tyr393/439) Antibody

The ABL1/2 (phospho-Tyr393/439) antibody is a specialized tool used to detect the phosphorylated forms of the ABL1 and ABL2 tyrosine kinases at specific tyrosine residues (Tyr393 in ABL1 and Tyr439 in ABL2). These non-receptor tyrosine kinases play critical roles in regulating cell proliferation, differentiation, adhesion, and stress responses. In their inactive state, ABL1/2 kinases adopt a autoinhibited conformation, where the SH3 and SH2 domains interact to suppress catalytic activity. Phosphorylation at Tyr393 (ABL1) or Tyr439 (ABL2) within the kinase activation loop is a key regulatory event that disrupts this autoinhibition, leading to kinase activation and downstream signaling. Dysregulation of ABL1/2 activity, particularly through mutations or chromosomal translocations (e.g., BCR-ABL1 in chronic myeloid leukemia), is implicated in oncogenesis. This antibody specifically recognizes the phosphorylated state of these residues, enabling researchers to study ABL1/2 activation status in cellular signaling pathways, disease models, or therapeutic contexts. It is widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to investigate ABL-mediated mechanisms in cancer, DNA damage response, and cytoskeletal dynamics. Validation typically includes testing in knockout cells or phospho-peptide competition assays to ensure specificity.