| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | H2B |
| Uniprot No | P33778 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-126aa |
| 氨基酸序列 | PEPSKSAPAPKKGSKKAITKAQKKDGKKRKRSRKESYSIYVYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK |
| 预测分子量 | 29.8kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于H2B重组蛋白的3篇参考文献的简要概括(注：以下内容为示例性虚构，实际文献需通过学术数据库检索)：
1. **文献名称**：*Expression and Purification of Recombinant Human Histone H2B in Escherichia coli*
**作者**：Zhang, L. et al.
**摘要**：本研究报道了一种高效表达和纯化重组人H2B组蛋白的方法，利用大肠杆菌表达系统，通过优化密码子和诱导条件提高蛋白产量，纯化的H2B可用于核小体重组及染色质结构研究。
2. **文献名称**：*Structural Insights into H2B Ubiquitination via Recombinant Protein Crystallography*
**作者**：Thompson, R. et al.
**摘要**：通过重组H2B与泛素连接酶复合物的共结晶实验，揭示了H2B泛素化修饰的关键位点及分子机制，为表观遗传调控中的染色质动态变化提供了结构基础。
3. **文献名称**：*Functional Analysis of Post-Translational Modifications on Recombinant H2B in Yeast*
**作者**：Suzuki, M. et al.
**摘要**：利用重组H2B蛋白在酵母模型中系统研究了磷酸化和乙酰化修饰对其在DNA修复和转录调控中的功能影响，证明特定修饰位点对基因组稳定性至关重要。
如需具体文献，建议在PubMed或Web of Science中检索关键词“recombinant H2B histone”或“H2B protein purification”。
Histone H2B is a core component of nucleosomes, the fundamental units of chromatin in eukaryotic cells. As one of the five major histone families (H1. H2A, H2B, H3. H4), H2B plays a critical role in DNA packaging and epigenetic regulation. It forms a heterodimer with H2A, which combines with H3-H4 tetramers to create the octameric core around which 147 base pairs of DNA are wrapped. Beyond structural support, H2B participates in DNA repair, replication, and transcriptional modulation through post-translational modifications (e.g., acetylation, ubiquitination) that influence chromatin accessibility.
Recombinant H2B proteins are engineered using expression systems (e.g., *E. coli*, yeast) to produce purified, sequence-identical histones for research applications. These proteins retain biological activity while offering batch-to-batch consistency, enabling standardized studies on nucleosome assembly, histone-DNA interactions, and chromatin remodeling mechanisms. Tagged versions (e.g., His, FLAG) facilitate purification and detection in assays. Recombinant H2B is particularly valuable for investigating histone crosstalk, epigenetic inheritance, and diseases linked to chromatin dysregulation, such as cancers and developmental disorders. Its use in reconstituted chromatin models has advanced our understanding of gene silencing, histone variant functions, and the impact of mutations on nucleosome stability.
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