首页 / 产品 / 蛋白 / 细胞因子、趋化因子与生长因子

Recombinant Human NRN1 protein

  • 中文名: 神经突蛋白1(NRN1)重组蛋白
  • 别    名: NRN1;NRN;Neuritin
货号: PA1000-5580
Price: ¥询价
数量:
大包装询价

产品详情

纯度>97%SDS-PAGE.
种属Human
靶点NRN1
Uniprot No Q9NPD7
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间28-115aa
氨基酸序列AGKCDAVFKG FSDCLLKLGD SMANYPQGLD DKTNIKTVCT YWEDFHSCTV TALTDCQEGA KDMWDKLRKE SKNLNIQGSL FELCGSGN
预测分子量 9.7 kDa
蛋白标签His tag N-Terminus
缓冲液PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是3篇关于NRN1(Neuritin 1)重组蛋白研究的参考文献示例(注:以下文献信息为模拟虚构,实际文献需通过学术数据库检索确认):

---

1. **文献名称**: "Expression and Purification of Recombinant NRN1 Protein in Escherichia coli for Neuronal Growth Studies"

**作者**: Smith A, et al.

**摘要**: 本研究报道了利用大肠杆菌系统高效表达重组NRN1蛋白的方法,通过His标签亲和层析纯化获得高纯度蛋白。功能实验表明,重组NRN1能显著促进体外培养神经元的突触生长和分支复杂度。

2. **文献名称**: "Neuritin 1 Recombinant Protein Attenuates Oxidative Stress-Induced Apoptosis in Cortical Neurons"

**作者**: Chen L, et al.

**摘要**: 作者在哺乳动物细胞中表达重组NRN1蛋白,验证其抑制氧化应激诱导的神经元凋亡的能力。结果显示,NRN1通过激活PI3K/Akt信号通路增强神经元存活,提示其潜在神经保护应用价值。

3. **文献名称**: "Structural and Functional Characterization of Recombinant Human NRN1 in a Mouse Model of Alzheimer’s Disease"

**作者**: Kim H, et al.

**摘要**: 该研究解析了重组人源NRN1蛋白的晶体结构,并在阿尔茨海默病小鼠模型中验证其功能。实验表明,NRN1重组蛋白可改善认知障碍并减少β-淀粉样蛋白沉积,为治疗神经退行性疾病提供新思路。

---

如需真实文献,建议在PubMed或Web of Science中检索关键词:**"Neuritin 1 recombinant protein"** 或 **"NRN1 recombinant expression"**。

背景信息

**Background of NRN1 Recombinant Protein**

Neuritin 1 (NRN1), also known as cpg15. is a small, secreted glycoprotein encoded by the *NRN1* gene, primarily recognized for its role in neural development and synaptic plasticity. It belongs to the neurotrophic factor family and is highly expressed in the brain, particularly in regions associated with learning and memory, such as the hippocampus and cerebral cortex. NRN1 promotes neurite outgrowth, dendritic arborization, and synaptic maturation, making it critical for neural circuit formation and repair. Its expression is activity-dependent, often upregulated by neuronal stimulation or neurotrophic factors like brain-derived neurotrophic factor (BDNF).

Structurally, NRN1 contains a conserved domain with glycosylphosphatidylinositol (GPI)-anchoring sites, allowing it to attach to cell membranes and interact with extracellular components. However, recombinant NRN1 is typically produced as a soluble, truncated form in heterologous expression systems (e.g., *E. coli* or mammalian cells), enabling studies of its extracellular signaling functions. Recombinant NRN1 retains bioactivity, activating downstream pathways such as PI3K-Akt and MAPK to support neuronal survival and connectivity.

Research highlights its therapeutic potential in neurodegenerative diseases (e.g., Alzheimer’s and Parkinson’s) and neural injury models, where it mitigates synaptic loss and enhances regeneration. Additionally, NRN1 is implicated in neurodevelopmental disorders and mood regulation, linking its dysfunction to conditions like depression. The production of recombinant NRN1 provides a tool for dissecting its mechanisms and exploring clinical applications, bridging gaps in understanding neurotrophin signaling and brain repair strategies.

客户数据及评论

折叠内容

大包装询价

×