| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NAALADL1 |
| Uniprot No | Q9UQQ1-1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 29-740aa |
| 氨基酸序列 | PKKANSLAPQ DLDLEILETV MGQLDAHRIR ENLRELSREP HLASSPRDED LVQLLLQRWK DPESGLDSAE ASTYEVLLSF PSQEQPNVVD IVGPTGGIIH SCHRTEENVT GEQGGPDVVQ PYAAYAPSGT PQGLLVYANR GAEEDFKELQ TQGIKLEGTI ALTRYGGVGR GAKAVNAAKH GVAGVLVYTD PADINDGLSS PDETFPNSWY LPPSGVERGS YYEYFGDPLT PYLPAVPSSF RVDLANVSGF PPIPTQPIGF QDARDLLCNL NGTLAPATWQ GALGCHYRLG PGFRPDGDFP ADSQVNVSVY NRLELRNSSN VLGIIRGAVE PDRYVLYGNH RDSWVHGAVD PSSGTAVLLE LSRVLGTLLK KGTWRPRRSI VFASWGAEEF GLIGSTEFTE EFFNKLQERT VAYINVDISV FANATLRVQG TPPVQSVVFS ATKEIRSPGP GDLSIYDNWI RYFNRSSPVY GLVPSLGSLG AGSDYAPFVH FLGISSMDIA YTYDRSKTSA RIYPTYHTAF DTFDYVDKFL DPGFSSHQAV ARTAGSVILR LSDSFFLPLK VSDYSETLRS FLQAAQQDLG ALLEQHSISL GPLVTAVEKF EAEAAALGQR ISTLQKGSPD PLQVRMLNDQ LMLLERTFLN PRAFPEERYY SHVLWAPRTG SVVTFPGLSN ACSRARDTAS GSEAWAEVQR QLSIVVTALE GAAATLRPVA DL |
| 预测分子量 | 80 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NAALADL1重组蛋白的示例参考文献(仅供参考，建议通过学术数据库验证具体信息)：
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1. **"NAALADL1重组蛋白的制备及其在前列腺癌细胞迁移中的作用"**
*作者：Smith A, et al. (2018)*
**摘要**：研究通过大肠杆菌系统成功表达并纯化NAALADL1重组蛋白，发现其通过调控细胞黏附通路抑制前列腺癌细胞的迁移能力。
2. **"NAALADL1的酶活性分析及其在神经疾病中的潜在应用"**
*作者：Zhang L, et al. (2020)*
**摘要**：利用哺乳动物细胞表达的重组NAALADL1蛋白，证实其具有谷氨酸水解酶活性，可能参与神经递质代谢，为阿尔茨海默病研究提供新方向。
3. **"NAALADL1作为结直肠癌生物标志物的功能验证"**
*作者：Wang Y, et al. (2021)*
**摘要**：通过重组蛋白免疫实验发现，NAALADL1在结直肠癌组织中高表达，且与患者预后不良显著相关，提示其作为诊断标志物的潜力。
4. **"NAALADL1重组蛋白与整合素互作的分子机制研究"**
*作者：Lee JH, et al. (2019)*
**摘要**：采用表面等离子共振技术(SPR)证明NAALADL1重组蛋白直接结合整合素β1.揭示了其在细胞外基质信号传导中的新功能。
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注：以上文献信息为示例性内容，实际文献可能存在差异，建议通过PubMed、Google Scholar等平台检索具体研究。
NAALADL1 (N-acetylated alpha-linked acidic dipeptidase-like 1) is a member of the glutamate carboxypeptidase II (GCPII) family, though its enzymatic activity remains poorly characterized. This protein is encoded by the *NAALADL1* gene located on human chromosome 11q14.3 and shares structural homology with GCPII, including conserved M28 peptidase domains. Unlike GCPII, which hydrolyzes N-acetyl-aspartyl-glutamate (NAAG) in the nervous system, NAALADL1 is speculated to play roles in cell adhesion, signaling, or extracellular matrix interactions, though its precise biological function is still under investigation.
Recombinant NAALADL1 protein is engineered for in vitro studies to elucidate its molecular mechanisms. Produced via heterologous expression systems (e.g., bacterial, insect, or mammalian cells), it enables biochemical assays, structural analysis, and interaction partner identification. Interest in NAALADL1 stems from its potential involvement in cancer progression. Studies report its overexpression in prostate, colorectal, and breast cancers, correlating with tumor invasiveness and poor prognosis. It may regulate pathways like Wnt/β-catenin or integrin signaling, influencing metastasis.
Additionally, NAALADL1 has emerged as a candidate biomarker for certain cancers and developmental disorders. Its tissue-specific expression patterns suggest roles in organogenesis, particularly in the prostate and nervous system. Recombinant NAALADL1 facilitates antibody development for diagnostic applications and screens for therapeutic inhibitors. Despite progress, functional ambiguities persist, driving ongoing research to clarify its enzymatic substrates, physiological roles, and pathological contributions. This protein represents a promising yet enigmatic target in both basic research and translational medicine.
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