| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Immunogen | Recombinant Protein |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于Phosphoserine(5B12)抗体的3篇参考文献及其简要概括：
1. **文献名称**：*"A monoclonal antibody specific for phosphoserine residues in proteins"*
**作者**：Smith A, Jones CD
**摘要**：该研究报道了单克隆抗体5B12的制备与特性分析，证明其对磷酸化丝氨酸残基具有高度特异性，适用于Western blot和免疫组化检测，且在多种细胞信号通路蛋白中验证了其应用潜力。
2. **文献名称**：*"Phosphoserine antibody 5B12 reveals dynamic phosphorylation patterns during mitosis"*
**作者**：Chen L, Wang H
**摘要**：利用5B12抗体研究有丝分裂过程中磷酸化丝氨酸的动态变化，发现其能特异性识别多种参与染色体分离的关键磷酸化蛋白，为细胞周期调控机制提供了新见解。
3. **文献名称**：*"Validation of 5B12 anti-phosphoserine antibody for neurodegenerative disease biomarker discovery"*
**作者**：Kim S, et al.
**摘要**：验证5B12抗体在脑脊液和神经组织样本中的适用性，发现其可高效检测tau蛋白等病理磷酸化标志物，支持其在阿尔茨海默病等神经退行性疾病研究中的应用价值。
(注：以上文献为示例，若需真实文献，建议通过PubMed或Google Scholar以关键词“Phosphoserine 5B12 antibody”进一步检索。)
Phosphoserine(5B12) antibody is a monoclonal antibody specifically designed to recognize serine residues phosphorylated at specific sites, a critical post-translational modification (PTM) involved in regulating protein function. Phosphorylation on serine residues plays a pivotal role in cellular signaling, apoptosis, cell cycle progression, and enzyme activation. The 5B12 clone targets phosphoserine epitopes, enabling researchers to study phosphorylation-dependent protein interactions, localization, and activity in various biological contexts.
Developed using immunogens containing phosphorylated serine motifs, this antibody exhibits high specificity for its target epitope while minimizing cross-reactivity with non-phosphorylated proteins or other phosphorylated amino acids (e.g., phosphothreonine or phosphotyrosine). It is widely utilized in techniques like western blotting, immunofluorescence, and immunoprecipitation to investigate signaling pathways in cancer, neurodegenerative diseases, and metabolic disorders.
The 5B12 antibody’s utility is validated through phosphatase-treated controls, where dephosphorylation abolishes signal detection, confirming its phosphorylation-specific binding. Its application has contributed to elucidating mechanisms in DNA damage response, kinase signaling cascades, and protein degradation pathways. Researchers often pair it with pan-specific antibodies to distinguish phosphorylated isoforms from total protein levels, enhancing experimental precision.
Overall, Phosphoserine(5B12) serves as a vital tool for exploring dynamic phosphorylation events, bridging molecular biology and disease mechanism studies.
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