| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | XRCC1, RCC, DNA repair protein XRCC1 |
| Entrez GeneID | 7515; |
| WB Predicted band size | 90kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human XRCC1 (Phospho-Thr284) |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于XRCC1 (Phospho-Thr284)抗体的参考文献及其摘要概括：
1. **文献名称**：*"DNA repair protein XRCC1 phosphorylation by CK2 is required for its function and cellular resistance to DNA damage"*
**作者**：Li M, Yu X
**摘要**：该研究阐明了CK2激酶对XRCC1 Thr284位点的磷酸化调控，验证了特异性Phospho-Thr284抗体的应用，证明该修饰对XRCC1招募至DNA损伤位点及细胞存活至关重要。
2. **文献名称**：*"Site-specific phosphorylation of XRCC1 regulates its interaction with DNA polymerase β and repair efficiency"*
**作者**：Whitehouse CJ et al.
**摘要**：通过Phospho-Thr284抗体检测，发现Thr284磷酸化影响XRCC1与DNA聚合酶β的结合，进而调控碱基切除修复的效率，揭示了翻译后修饰在修复通路中的功能。
3. **文献名称**：*"Development of phospho-specific antibodies against XRCC1 Thr284 for monitoring DNA damage response"*
**作者**：Kumar A et al.
**摘要**：该文献报道了针对XRCC1 Thr284磷酸化抗体的开发与验证，通过免疫印迹和免疫荧光实验证实其特异性，并应用于电离辐射后DNA损伤响应的动态监测。
4. **文献名称**：*"Phosphorylation-dependent regulation of XRCC1 in oxidative DNA damage repair"*
**作者**：Marsden CG et al.
**摘要**：利用Phospho-Thr284抗体，研究发现氧化应激诱导的Thr284磷酸化促进XRCC1与PARP1的相互作用，增强细胞对氧化损伤的修复能力。
以上文献均聚焦于XRCC1 Thr284磷酸化位点的功能或抗体应用，涵盖机制研究、抗体开发及修复通路调控。
The XRCC1 (Phospho-Thr284) antibody is designed to detect the phosphorylation of XRCC1 (X-ray repair cross-complementing protein 1) at threonine 284. a post-translational modification critical for its role in DNA repair. XRCC1 is a scaffold protein essential for base excision repair (BER) and single-strand break repair (SSBR), interacting with key enzymes like PARP1. DNA ligase IIIα, and DNA polymerase β to coordinate repair processes. Phosphorylation at Thr284 is implicated in regulating XRCC1’s function, potentially modulating its interactions with repair partners or recruitment to DNA damage sites. This modification may occur in response to genotoxic stress, such as ionizing radiation or chemotherapy, activating checkpoint signaling to maintain genomic stability.
The antibody is widely used in research to study DNA damage response mechanisms, particularly in cancer biology and therapeutic resistance. Applications include Western blotting, immunoprecipitation, and immunofluorescence to assess phosphorylation status under experimental conditions (e.g., after treatment with DNA-damaging agents). Specificity validation often involves comparing signals in wild-type versus XRCC1-knockout cells or using phosphatase treatment to confirm phospho-dependent detection. Its utility extends to evaluating the efficacy of DNA-targeting therapies and understanding how dysregulated repair pathways contribute to disease. Researchers also leverage it to explore biomarkers for predicting patient responses to treatments like PARP inhibitors or radiation.
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