Rabbit Polyclonal MKI67IP(phospho-Thr234) Antibody

The MKI67IP (phospho-Thr234) antibody is a specialized tool for studying post-translational modifications of the MKI67IP protein, also known as BIRC5BP or Survivin-2B. MKI67IP is a nuclear antigen-binding protein that interacts with Survivin, a key regulator of mitosis and apoptosis. Its phosphorylation at threonine 234 (Thr234) is implicated in cell cycle regulation, particularly during the G2/M phase transition. This modification likely modulates MKI67IP’s interaction with Survivin or other components of the chromosomal passenger complex, influencing mitotic progression, spindle assembly, or apoptosis evasion. The phospho-Thr234-specific antibody enables researchers to detect and quantify this activation state in cellular models, offering insights into dynamic cell cycle events or dysregulation in pathological contexts. Applications include immunofluorescence, Western blotting, and IHC to map spatial-temporal phosphorylation patterns in tissues or cultured cells. Studies using this antibody may explore its role in cancer biology, as Survivin-related proteins are often overexpressed in tumors. Validation typically involves knockout/knockdown controls or phosphatase treatment to confirm phosphorylation-dependent signals. Proper storage and optimized dilution protocols are critical for maintaining antibody specificity.