| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/300 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | MAPKAPK1A; RSK1; Ribosomal protein S6 kinase alpha-1; S6K-alpha-1; 90 kDa ribosomal protein S6 kinase 1; p90-RSK 1; p90RSK1; p90S6K; MAP kinase-activated protein kinase 1a; MAPK-activated protein kinase 1a; MAPKAP kinase 1a; MAPKAPK-1a; Ribosomal S6 kinase 1; RSK-1; MAPKAPK1C; RSK3; Ribosomal protein S6 kinase alpha-2; S6K-alpha-2; 90 kDa ribosomal protein S6 kinase 2; p90-RSK 2; p90RSK2; MAP kinase-activated protein kinase 1c; MAPK-activated protein kinase 1c; MAPKAP kinase 1c; MAPKAPK-1c; Ribosomal S6 kinase 3; RSK-3; pp90RSK3; ISPK1; MAPKAPK1B; RSK2; Ribosomal protein S6 kinase alpha-3; S6K-alpha-3; 90 kDa ribosomal protein S6 kinase 3; p90-RSK 3; p90RSK3; Insulin-stimulated protein kinase 1; ISPK-1; MAP kinase-activated protein kinase 1b; MAPK-activated protein kinase 1b; MAPKAP kinase 1b; MAPKAPK-1b; Ribosomal S6 kinase 2; RSK-2; pp90RSK2 |
| Entrez GeneID | 6195; |
| WB Predicted band size | 90kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human RSK1/2/3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于 RSK1/2/3 (phospho-Thr573/570/577) 抗体的参考文献示例(文献信息为模拟生成，仅供参考)：
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1. **文献名称**: "ERK-dependent phosphorylation of the kinase RSK regulates mitotic progression"
**作者**: Frödin M, et al.
**摘要**: 研究揭示了 ERK1/2 对 RSK 家族成员(RSK1/2/3)在 Thr573/570/577 位点的磷酸化调控机制，并利用特异性抗体证明该磷酸化事件在细胞周期有丝分裂中的关键作用。
2. **文献名称**: "RSK-mediated survival signaling through phosphorylation of the pro-apoptotic protein BAD"
**作者**: Smith JA, et al.
**摘要**: 通过磷酸化特异性抗体检测，证实 RSK1/2/3 在 Thr573/570/577 位点的激活通过磷酸化 BAD 蛋白抑制细胞凋亡，促进细胞存活。
3. **文献名称**: "Role of p90 RSK in cancer: Phosphoproteomic analysis reveals novel substrates"
**作者**: Jensen CJ, et al.
**摘要**: 利用抗 phospho-Thr573/570/577 抗体进行蛋白质组学分析，发现 RSK 在肿瘤中异常激活并通过磷酸化下游靶标(如 CREB)驱动癌症进展。
4. **文献名称**: "A RSK isoform-specific signaling network controlling cell motility"
**作者**: Anjum R, Blenis J.
**摘要**: 研究比较了 RSK1/2/3 的 Thr 磷酸化差异，使用特异性抗体阐明不同亚型在细胞迁移中的独特功能，并揭示其与 MAPK 通路的动态互作。
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注：以上文献信息为示例，实际引用需查询 PubMed 或学术数据库获取真实文献。
The RSK1/2/3 (phospho-Thr573/570/577) antibody detects the activated forms of the RSK (ribosomal S6 kinase) family members (RSK1. RSK2. RSK3) when phosphorylated at specific threonine residues (Thr573 in RSK1. Thr570 in RSK2. Thr577 in RSK3). These kinases are downstream effectors of the ERK/MAPK signaling pathway and play critical roles in cell survival, proliferation, and differentiation. Activation of RSK occurs via a sequential phosphorylation cascade: ERK1/2 first phosphorylates RSK at the C-terminal kinase domain, triggering autophosphorylation and subsequent phosphorylation of the N-terminal kinase domain by PDK1. The phospho-Thr573/570/577 sites reside within the activation loop of the N-terminal kinase domain, essential for full enzymatic activity.
This antibody is widely used to study RSK activation in response to growth factors, hormones, or cellular stress. It helps researchers assess pathway activity in diseases like cancer, where RSK signaling is often dysregulated. Specificity for the phosphorylated forms allows differentiation between active and inactive RSK isoforms, making it valuable in Western blotting, immunofluorescence, and immunohistochemistry. Proper controls, such as phosphorylation-blocking peptides or phosphatase-treated samples, are recommended to confirm signal specificity. Understanding RSK phosphorylation dynamics aids in exploring therapeutic targets for conditions involving aberrant MAPK/ERK signaling.
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