| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/300 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human MNDA |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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1. **"Protein kinase Cι/ζ phosphorylates the β1-integrin cytoplasmic domain at Thr41/Thr42 to promote cell migration"**
- **作者**: Zhang et al.
- **摘要**: 该研究揭示了PKCι/ζ通过磷酸化β1整合素胞内域的Thr41/Thr42位点，调控细胞迁移和侵袭的分子机制，并利用特异性抗体验证了磷酸化位点的功能相关性。
2. **"Phosphorylation of PKCζ at Thr410 regulates its kinase activity and substrate specificity in epithelial polarity"**
- **作者**: Suzuki et al.
- **摘要**: 文章通过磷酸化特异性抗体证实，PKCζ在Thr410位点的磷酸化是其激酶活性和细胞极性建立的关键调控步骤，并影响下游底物Par3的募集。
3. **"A novel role for PKCι in TGF-β signaling: Phosphorylation of Smad3 at Thr412 mediates epithelial-mesenchymal transition"**
- **作者**: Wang et al.
- **摘要**: 研究发现PKCι通过磷酸化Smad3的Thr412位点，促进TGF-β诱导的上皮-间质转化(EMT)，相关抗体被用于检测磷酸化状态及功能验证。
4. **"Targeting phospho-PKCι/ζ signaling disrupts oncogenic growth in non-small cell lung cancer"**
- **作者**: Regala et al.
- **摘要**: 该文献利用磷酸化抗体证明PKCι/ζ在Thr412/Thr410位点的异常激活与肺癌细胞增殖和存活相关，并探索了其作为治疗靶点的潜力。
(注：上述文献为示例，实际引用需根据具体研究补充真实发表信息。)
The PKC iota/zeta (phospho-Thr412/410) antibody is designed to detect the activated forms of protein kinase C (PKC) isoforms ι (iota) and ζ (zeta), specifically phosphorylated at conserved threonine residues (Thr412 in PKCι and Thr410 in PKCζ). These atypical PKC isoforms belong to the PKC family of serine/threonine kinases, which regulate diverse cellular processes, including cell survival, proliferation, and polarity. Unlike conventional PKCs, atypical PKCs (aPKCs) are not dependent on calcium or diacylglycerol for activation but instead rely on phosphorylation and protein-protein interactions. Phosphorylation at Thr412/410 is critical for their catalytic activity, serving as a hallmark of kinase activation. This phosphorylation event is mediated by the phosphoinositide-dependent kinase 1 (PDK1) during signaling cascades involving growth factors, cytokines, or cellular stress.
The PKC iota/zeta (phospho-Thr412/410) antibody is widely used in research to study the role of activated aPKCs in pathways such as NF-κB signaling, mTORC2-mediated cell survival, and polarized cell migration. Dysregulation of PKCι/ζ has been implicated in cancer, neurodegenerative diseases, and immune disorders. For example, PKCι is overexpressed in lung and pancreatic cancers, while PKCζ modulates inflammatory responses. This antibody aids in detecting activated PKCι/ζ in techniques like Western blotting, immunofluorescence, or immunohistochemistry, providing insights into their spatial and temporal activation under physiological or pathological conditions.
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