| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/300 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ETV6; Tel; TEL oncogene; TEL/ABL; TEL1;;ETV6 |
| WB Predicted band size | 53 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human ETV6 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇涉及TPL2 (phospho-Thr290)抗体的相关文献摘要：
1. **文献名称**: *"Phosphorylation of TPL2 at Thr290 regulates its interaction with NF-κB1/p105"*
**作者**: Cho et al.
**摘要**: 研究通过磷酸化特异性抗体证实，TLR信号通路诱导的TPL2 Thr290磷酸化促进其与NF-κB1/p105的解离，从而激活下游MAPK通路，揭示了炎症反应中TPL2的调控机制。
2. **文献名称**: *"Role of TPL2 kinase activity in colitis-associated cancer"*
**作者**: Gantke et al.
**摘要**: 利用phospho-Thr290抗体发现，小鼠结肠炎模型中TPL2的Thr290磷酸化水平升高，通过促进ERK信号驱动肿瘤发生，提示其作为结直肠癌潜在治疗靶点。
3. **文献名称**: *"Mechanistic insights into TPL2 activation by phosphorylation"*
**作者**: Roget et al.
**摘要**: 通过体外激酶实验结合磷酸化抗体检测，证明Thr290磷酸化是TPL2激酶完全激活的必要条件，并解析了其构象变化对底物识别的影响。
4. **文献名称**: *"TPL2 phosphorylation status modulates macrophage polarization in sepsis"*
**作者**: Wang et al.
**摘要**: 在脓毒症模型中，使用phospho-Thr290抗体检测发现，抑制TPL2磷酸化可减少促炎型巨噬细胞分化，为免疫过度激活提供了干预策略。
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**注**：以上文献信息为示例性概括，实际文献可能需要通过PubMed或Web of Science以关键词“TPL2 phospho-Thr290 antibody”检索确认。部分研究可能侧重机制而非抗体应用细节。
The TPL2 (phospho-Thr290) antibody is a specialized tool used to detect the activated form of TPL2 kinase (tumor progression locus 2), also known as MAP3K8 or COT. TPL2 is a serine/threonine kinase involved in regulating inflammatory responses, cell proliferation, and stress-activated pathways, primarily through the MAPK signaling cascade. Phosphorylation at threonine 290 (Thr290) is critical for TPL2 activation, as it triggers conformational changes that release the kinase from its inhibitory binding partner ABIN2 or p105 (NF-κB precursor), enabling downstream signaling to targets like MEK1/2 and ERK1/2. This phosphorylation event is often associated with stimuli such as cytokines (e.g., TNF-α, IL-1β), Toll-like receptor agonists, or cellular stress.
The TPL2 (phospho-Thr290) antibody is widely used in research to study TPL2 activation dynamics in immune cells, cancer models, and inflammatory diseases. It helps identify contexts where TPL2-driven pathways contribute to pathological states, including autoimmune disorders, infections, and tumorigenesis. Validated applications include Western blotting, immunoprecipitation, and immunohistochemistry, often in combination with inhibitors or genetic models to dissect TPL2's role in signaling networks. Its specificity for the phosphorylated form makes it valuable for distinguishing active versus inactive TPL2 in experimental systems, offering insights into therapeutic targeting of this kinase in disease.
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