| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | BHLHE75; SRC2; TIF2; Nuclear receptor coactivator 2; NCoA-2; Class E basic helix-loop-helix protein 75; bHLHe75; Transcriptional intermediary factor 2; hTIF2 |
| Entrez GeneID | 10499; |
| WB Predicted band size | 160kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human NCOA2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于NCOA2 (phospho-Ser736)抗体的3篇参考文献示例(注：文献内容为模拟概括，仅供参考)：
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1. **文献名称**：*Phosphorylation of NCOA2 at Serine 736 by MAPK Signaling Regulates Transcriptional Activation*
**作者**：Li Y, et al.
**摘要**：该研究通过质谱分析和体外激酶实验，发现丝裂原活化蛋白激酶(MAPK)信号通路可磷酸化NCOA2的Ser736位点。研究使用特异性抗磷酸化Ser736抗体验证了该修饰在细胞核定位和激素依赖性转录激活中的关键作用。
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2. **文献名称**：*Development and Validation of a Phospho-Specific Antibody for NCOA2 Ser736 in Breast Cancer Models*
**作者**：Smith J, et al.
**摘要**：本文报道了一种针对NCOA2 Ser736磷酸化位点的单克隆抗体的开发与验证。通过Western blot和免疫荧光实验，证明该抗体可特异性识别雌激素受体阳性乳腺癌细胞中NCOA2的激活状态，并与肿瘤增殖相关。
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3. **文献名称**：*NCOA2 Phosphorylation Modulates Coactivator Complex Assembly in Prostate Cancer*
**作者**：Chen R, et al.
**摘要**：研究利用NCOA2 (phospho-Ser736)抗体，揭示了雄激素受体(AR)信号通路诱导的磷酸化通过改变NCOA2与组蛋白乙酰转移酶p300的相互作用，促进前列腺癌细胞中致癌基因的转录调控。
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**说明**：以上文献为示例性质，实际研究中请通过PubMed或Web of Science以关键词“NCOA2 phospho-Ser736 antibody”或“NCOA2 phosphorylation”检索最新文献。部分相关研究可能涉及抗体应用、信号通路机制或疾病关联。
The NCOA2 (nuclear receptor coactivator 2), also known as GRIP1 or TIF2. is a transcriptional coactivator that interacts with nuclear receptors and other transcription factors to regulate gene expression. Phosphorylation at specific residues, such as Ser736. modulates its activity, stability, and protein-protein interactions. The NCOA2 (phospho-Ser736) antibody is a specialized tool designed to detect the phosphorylated form of NCOA2 at serine 736. a post-translational modification linked to cellular signaling pathways. This phosphorylation event is implicated in enhancing NCOA2’s coactivator function, particularly in hormone-dependent signaling, including pathways activated by estrogen, androgen, and other steroid receptors.
Studies suggest that Ser736 phosphorylation may influence NCOA2’s role in cancer progression, such as in breast or prostate cancer, where it promotes cell proliferation and survival. The antibody is widely used in techniques like Western blotting, immunohistochemistry, or immunofluorescence to study NCOA2 activation dynamics in response to extracellular signals (e.g., growth factors, hormones) or under pathological conditions. Researchers also employ it to explore how kinase pathways (e.g., MAPK, AKT) regulate NCOA2 activity and its crosstalk with oncogenic processes. By targeting this specific phosphorylation site, the antibody helps elucidate mechanisms underlying transcriptional deregulation in diseases and potential therapeutic targeting of coactivator-driven pathways.
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