| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | AKT1 |
| Entrez GeneID | 207; |
| WB Predicted band size | 60kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic Peptide |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于Phospho-Akt (S473) Mouse (6F8)抗体的示例参考文献(注：文献为示例性概括，实际文献请通过PubMed或抗体制造商官网查询)：
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1. **文献名称**：*"Role of Akt phosphorylation in prostate cancer cell survival"*
**作者**：Smith et al.
**摘要**：研究利用6F8抗体检测Akt (S473)磷酸化水平，发现其在雄激素依赖性前列腺癌细胞中显著升高，提示磷酸化Akt通过抑制凋亡促进肿瘤生长。
2. **文献名称**：*"Validation of Phospho-Akt (6F8) antibody in glioblastoma models"*
**作者**：Lee & Park
**摘要**：验证6F8抗体在胶质母细胞瘤中的特异性，证实其可用于Western blot和免疫荧光，并显示PI3K抑制剂可降低S473位点磷酸化。
3. **文献名称**：*"Akt activation in diabetes-associated breast cancer"*
**作者**：Chen et al.
**摘要**：通过6F8抗体分析糖尿病患者的乳腺癌样本，发现高血糖通过mTORC2增强Akt (S473)磷酸化，促进肿瘤侵袭。
4. **文献名称**：*"Antibody specificity profiling for Akt pathway studies"*
**作者**：Johnson et al.
**摘要**：比较多种Akt磷酸化抗体，指出6F8抗体在人和小鼠组织中的高特异性，适用于体内外信号通路动态分析。
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建议通过**PubMed**或抗体生产商(如Cell Signaling Technology、Abcam)的产品说明书获取真实文献。
The Phospho-Akt (S473) Mouse monoclonal antibody (clone 6F8) is a widely used reagent in molecular biology research to detect Akt (protein kinase B) phosphorylated at serine residue 473. a critical post-translational modification associated with its activation. Akt plays a central role in the PI3K/Akt/mTOR signaling pathway, regulating processes like cell survival, proliferation, metabolism, and apoptosis. Phosphorylation at S473. often in conjunction with Thr308. is required for full Akt activation, typically triggered by growth factors, hormones, or cellular stress.
Clone 6F8 is a mouse-derived IgG1 antibody generated using a synthetic phosphopeptide corresponding to residues surrounding S473 in human Akt1. It exhibits specificity for the phosphorylated S473 epitope across Akt isoforms (Akt1. Akt2. Akt3) in humans, mice, and rats, making it valuable for applications such as Western blotting, immunoprecipitation, and immunohistochemistry. Researchers frequently use this antibody to study oncogenic signaling, insulin resistance, or therapeutic responses in cancers and metabolic disorders, as hyperactivation of Akt is common in these conditions. Validation typically includes knockout controls or phosphatase treatment to confirm phosphorylation-dependent detection. Its reliability has established it as a staple tool for probing Akt activation status in diverse experimental models.
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