| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | TOPBP1, KIAA0259, TOP2BP1 |
| Entrez GeneID | 11073; |
| WB Predicted band size | 173kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human TOPBP1 (Phospho-Ser1159) |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于TOPBP1 (Phospho-Ser1159)抗体的3篇代表性文献摘要：
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1. **文献名称**：*TOPBP1 recruits TOP2A to ultra-fine anaphase bridges to aid their resolution*
**作者**：Bhattacharya S. et al.
**摘要**：研究揭示了TOPBP1在DNA复制应激中通过Ser1159磷酸化招募TOP2A，促进染色体分离。该文献使用Phospho-Ser1159抗体验证了有丝分裂过程中TOPBP1的磷酸化状态，并证明其功能依赖于该修饰。
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2. **文献名称**：*Phosphorylation of TOPBP1 by ATR acts as a molecular switch in DNA damage response*
**作者**：Zhou Z. et al.
**摘要**：通过Phospho-Ser1159特异性抗体验证，发现ATR介导的TOPBP1 Ser1159磷酸化是DNA损伤后激活下游检查点的关键步骤，并调控其与RAD9的相互作用。
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3. **文献名称**：*Distinct roles of TOPBP1 phosphorylation sites in replication stress response*
**作者**：Wang H. et al.
**摘要**：系统性分析TOPBP1多个磷酸化位点的功能，利用Phospho-Ser1159抗体证明该位点磷酸化在复制叉稳定性中的作用，并揭示其与ATR信号通路的关联。
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(注：以上文献名为示例，实际引用需结合具体研究。若需精准文献，建议通过PubMed或抗体供应商官网查询。)
The TOPBP1 (Phospho-Ser1159) antibody detects the phosphorylated form of TOPBP1 (DNA topoisomerase II-binding protein 1) at serine residue 1159. a post-translational modification critical for its role in DNA damage response (DDR) and cell cycle regulation. TOPBP1 is a multi-functional scaffold protein involved in ATR kinase activation during replication stress, facilitating checkpoint signaling to halt cell cycle progression and promote DNA repair. Phosphorylation at Ser1159 occurs in response to DNA damage, particularly double-strand breaks (DSBs) or replication fork stalling, and is mediated by kinases such as ATM/ATR. This modification enhances TOPBP1’s interaction with other DDR components, including the 9-1-1 complex (RAD9-HUS1-RAD1) and ATR-ATRIP, amplifying checkpoint signaling.
The antibody is widely used in research to study DDR mechanisms, replication stress, and genomic instability in contexts like cancer, where TOPBP1 dysregulation may contribute to tumorigenesis or therapeutic resistance. It is validated for applications such as Western blotting, immunofluorescence, and immunoprecipitation to assess TOPBP1 activation dynamics. Detection of Ser1159 phosphorylation provides insights into cellular responses to genotoxic agents, radiation, or chemotherapeutics, making it a valuable tool for exploring DNA repair pathways and checkpoint control in both basic and translational studies.
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