Rabbit Polyclonal 5-Lipoxygenase(phospho-Ser272) Antibody

The 5-Lipoxygenase (5-LOX) enzyme plays a pivotal role in the biosynthesis of pro-inflammatory lipid mediators called leukotrienes, which are implicated in inflammatory disorders, cancer, and cardiovascular diseases. The phosphorylation of 5-LOX at serine 272 (Ser272) is a critical post-translational modification regulating its enzymatic activity, subcellular localization, and interaction with co-factors like 5-lipoxygenase-activating protein (FLAP). This phosphorylation event, mediated by signaling pathways such as MAPK or PKC, enhances 5-LOX membrane translocation and promotes leukotriene synthesis by stabilizing its active conformation. Antibodies specific to 5-LOX phosphorylated at Ser272 (phospho-Ser272) are essential tools for studying the activation status and regulatory mechanisms of 5-LOX in physiological and pathological contexts. These antibodies enable researchers to detect phosphorylated 5-LOX in cellular models, tissues, or biofluids using techniques like Western blotting, immunofluorescence, or immunohistochemistry. Their application is particularly valuable in investigating inflammatory responses, evaluating drug efficacy targeting 5-LOX pathways, or exploring disease mechanisms linked to dysregulated leukotriene production. Validation of such antibodies typically includes testing in phosphorylation-deficient mutants and verifying specificity across relevant cell types. Understanding 5-LOX (phospho-Ser272) dynamics provides insights into therapeutic strategies for conditions like asthma, atherosclerosis, or cancer.