| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Entrez GeneID | 816; |
| WB Predicted band size | 50kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthetic Peptide |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于CaMKIIβ/γ/δ(Phospho Thr287)(4H2)抗体的3篇模拟参考文献(内容基于领域知识虚构，仅供参考)：
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1. **文献名称**：*Phosphorylation of CaMKIIβ at Thr287 regulates dendritic spine plasticity in hippocampal neurons*
**作者**：Zhang L, et al.
**摘要**：本研究利用4H2抗体检测海马神经元中CaMKIIβ亚型Thr287位点的磷酸化水平，发现该位点的磷酸化与突触可塑性和长时程增强(LTP)密切相关，为学习记忆的分子机制提供了新证据。
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2. **文献名称**：*CaMKIIγ phosphorylation at Thr287 promotes cardiac hypertrophy via NF-κB activation*
**作者**：Wang Y, et al.
**摘要**：通过4H2抗体的Western blot分析，揭示了压力超负荷心肌细胞中CaMKIIγ亚型Thr287磷酸化水平升高，激活NF-κB通路并驱动病理性心肌肥厚，提示其作为心力衰竭治疗的潜在靶点。
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3. **文献名称**：*Differential roles of CaMKIIδ isoforms in cancer metastasis: Insights from phospho-specific antibody 4H2*
**作者**：Chen R, et al.
**摘要**：采用4H2抗体检测肿瘤组织中CaMKIIδ亚型的Thr287磷酸化状态，发现高磷酸化水平与肿瘤细胞侵袭转移能力增强相关，机制涉及上皮-间质转化(EMT)的调控。
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**注**：以上文献为示例性内容，实际引用需通过PubMed或学术数据库检索真实研究。若需具体文章，可提供关键词协助筛选。
The CaMKIIβ/γ/δ (Phospho-Thr287) (4H2) antibody is a monoclonal antibody specifically designed to detect the phosphorylated form of calcium/calmodulin-dependent protein kinase II (CaMKII) isoforms β, γ, and δ at threonine residue 287. CaMKII is a multifunctional serine/threonine kinase critical for calcium-mediated signaling pathways, regulating processes such as synaptic plasticity, gene expression, and muscle contraction. Autophosphorylation at Thr287 (Thr286 in CaMKIIα) is a key regulatory event that sustains kinase activity even after calcium levels decline, enabling long-term signaling effects.
The 4H2 clone recognizes this conserved phosphorylation site across CaMKIIβ, γ, and δ isoforms, making it a valuable tool for studying their activation in diverse tissues, including the brain, heart, and skeletal muscle. This antibody is widely used in neuroscience and cardiovascular research to investigate CaMKII’s role in conditions like cardiac hypertrophy, arrhythmias, and neurological disorders. Its applications include Western blotting, immunohistochemistry, and immunofluorescence, often paired with total CaMKII antibodies to assess activation ratios. Validation typically involves knockout controls or phosphatase treatment to confirm phosphorylation-dependent specificity. Researchers favor this antibody for its reliability in probing dynamic CaMKII signaling in disease models or pharmacological studies.
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