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Mouse Monoclonal Phospho-Akt(S473)Mouse(7F9) Antibody

  • 中文名: Phospho-Akt (S473) Mouse(7F9)抗体
  • 别    名: AKT1
货号: IPDX41198
Price: ¥1280
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/100-1/200 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesAKT1
Entrez GeneID207;
WB Predicted band size60kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenSynthetic Peptide
FormulationPurified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol.

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参考文献

以下是关于Phospho-Akt (S473) Mouse (7F9)抗体的示例参考文献(内容为虚构示例,建议通过学术数据库查询真实文献):

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1. **"Akt activation in tumorigenesis: Role of Ser473 phosphorylation"**

*Author: Smith J et al.*

**摘要**:通过Western blot和免疫组化分析,使用Phospho-Akt (S473) 7F9抗体,揭示乳腺癌中Akt的S473磷酸化水平与肿瘤转移和耐药性显著相关。

2. **"Validation of phospho-specific Akt antibodies in signaling studies"**

*Author: Lee H et al.*

**摘要**:系统性评估多种Akt磷酸化抗体,证实7F9克隆在检测细胞凋亡模型中Akt激活的特异性,适用于流式细胞术和免疫荧光。

3. **"Insulin-induced Akt phosphorylation in diabetic models"**

*Author: Chen R et al.*

**摘要**:利用该抗体在小鼠肌肉组织中检测胰岛素刺激后Akt S473的动态磷酸化变化,阐明代谢调控中PI3K/Akt通路的关键作用。

4. **"Targeting Akt in glioblastoma therapy resistance"**

*Author: Wang Y et al.*

**摘要**:通过7F9抗体验证放疗后胶质母细胞瘤中Akt异常激活,揭示其与DNA修复蛋白的相互作用,为联合靶向治疗提供依据。

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**提示**:实际文献需通过PubMed或Google Scholar搜索关键词“Phospho-Akt Ser473 7F9”或抗体货号(如#XXXX),结合研究领域筛选。抗体厂商官网(如CST、Abcam)通常也会提供相关引用文献列表。

背景信息

The Phospho-Akt (S473) Mouse (7F9) monoclonal antibody is a widely used reagent for detecting Akt phosphorylated at serine residue 473. a key post-translational modification associated with Akt activation. Akt (protein kinase B) is a central regulator of cell survival, proliferation, and metabolism, functioning within the PI3K/Akt/mTOR signaling pathway. Phosphorylation at S473. along with T308. is required for full Akt activation, typically triggered by growth factors, insulin, or other stimuli. The 7F9 antibody, generated in mice through hybridoma technology, specifically recognizes the phosphorylated S473 epitope in human, mouse, and rat Akt isoforms (Akt1. Akt2. Akt3). Its specificity makes it valuable for applications like Western blotting, immunofluorescence, and immunohistochemistry to study Akt activation in cancer, diabetes, and neurodegenerative diseases. Researchers frequently employ this antibody to investigate dysregulated PI3K/Akt signaling in tumors, as hyperactivation of Akt via S473 phosphorylation is linked to oncogenesis and therapy resistance. Validation typically includes testing in knockout models or with phosphatase-treated lysates to confirm phospho-specificity. Proper controls are essential to distinguish between phosphorylated and total Akt levels in experimental settings.

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