Rabbit Polyclonal EPHA2(phospho-Tyr588/596) Antibody

The EPHA2 (phospho-Tyr588/596) antibody detects endogenous levels of EPHA2 protein when phosphorylated at tyrosine residues 588 and 596. EPHA2. a member of the Eph receptor tyrosine kinase family, plays critical roles in cell-cell communication, tissue patterning, and cytoskeletal dynamics. Unlike other Eph receptors typically activated by Ephrin ligands, EPHA2 can exhibit ligand-independent signaling in cancer contexts, contributing to tumor progression, metastasis, and angiogenesis. Phosphorylation at Tyr588 and Tyr596 occurs in the kinase domain activation loop, a key step in receptor activation. This post-translational modification enhances EPHA2 kinase activity and initiates downstream signaling cascades, including MAPK/ERK and PI3K/AKT pathways. Aberrant EPHA2 phosphorylation is associated with increased cell proliferation, migration, and therapeutic resistance in various cancers (e.g., breast, lung, melanoma). The EPHA2 (phospho-Tyr588/596) antibody is widely used in cancer research to study receptor activation status, particularly in pathological conditions where EPHA2 signaling is dysregulated. It serves as a valuable tool for Western blotting, immunohistochemistry, and immunofluorescence applications, enabling researchers to investigate EPHA2's role in tumor biology and evaluate its potential as a therapeutic target or diagnostic biomarker. Validation typically includes testing in cell lines with known phosphorylation status or following Ephrin-A1 ligand stimulation.