关键信息
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基因名
KCNJ2
- 应用
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别名
KCNJ2; IRK1; Inward rectifier potassium channel 2; Cardiac inward rectifier potassium channel; Inward rectifier K(+ channel Kir2.1; IRK-1; hIRK1
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P63252
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表达区间
328-427aa
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氨基酸序列
PVLFEEKHYYKVDYSRFHKTYEVPNTPLCSARDLAEKKYILSNANSFCYENEVALTSKEEDDSENGVPESTSTDTPPDIDLHNQASVPLEPRPLRRESEI
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分子量
36.74 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
KCNJ2, also known as the inwardly rectifying potassium channel 2, is a critical protein encoded by the KCNJ2 gene, primarily involved in regulating the electrical activity of excitable cells such as neurons and cardiac myocytes. Its primary function is to maintain the resting membrane potential and contribute to the repolarization phase of action potentials by allowing potassium ions to flow into the cell more easily than they can flow out. Mutations in KCNJ2 are linked to a number of inherited conditions, most notably Andersen-Tawil syndrome, which is characterized by periodic paralysis, cardiac arrhythmias, and developmental issues. Understanding the structure and function of KCNJ2 is vital for elucidating its role in these pathologies and for the development of potential therapeutic interventions. Recent advances in recombinant protein technology have enabled researchers to produce and characterize KCNJ2 proteins in controlled conditions, allowing for detailed studies of their biophysical properties and interaction with pharmacological agents. The investigation of KCNJ2 not only deepens our insight into ion channel function but also paves the way for novel strategies to treat related disorders, making it a significant target in cardiovascular and neurological research.
