关键信息
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基因名
KCNJ4
- 应用
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别名
KCNJ4; IRK3; Inward rectifier potassium channel 4; HIRK2; HRK1; Hippocampal inward rectifier; HIR; Inward rectifier K(+ channel Kir2.3; IRK-3; Potassium channel. inwardly rectifying subfamily J member 4
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P48050
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表达区间
1-445aa
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氨基酸序列
MHGHSRNGQAHVPRRKRRNRFVKKNGQCNVYFANLSNKSQRYMADIFTTCVDTRWRYMLMIFSAAFLVSWLFFGLLFWCIAFFHGDLEASPGVPAAGGPAAGGGGAAPVAPKPCIMHVNGFLGAFLFSVETQTTIGYGFRCVTEECPLAVIAVVVQSIVGCVIDSFMIGTIMAKMARPKKRAQTLLFSHHAVISVRDGKLCLMWRVGNLRKSHIVEAHVRAQLIKPYMTQEGEYLPLDQRDLNVGYDIGLDRIFLVSPIIIVHEIDEDSPLYGMGKEELESEDFEIVVILEGMVEATAMTTQARSSYLASEILWGHRFEPVVFEEKSHYKVDYSRFHKTYEVAGTPCCSARELQESKITVLPAPPPPPSAFCYENELALMSQEEEEMEEEAAAAAAVAAGLGLEAGSKEEAGIIRMLEFGSHLDLERMQASLPLDNISYRRESAI
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分子量
75.35 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
KCNJ4, also known as the inwardly rectifying potassium channel 4, is a member of the KCNJ family, which plays a crucial role in regulating cellular excitability and maintaining ion balance in various tissues. This protein is primarily expressed in the heart and brain, where it contributes to the stabilization of resting membrane potential and the modulation of action potentials. Research on KCNJ4 has gained significance due to its involvement in various physiological processes and potential pathological conditions. Alterations in KCNJ4 function have been linked to cardiac arrhythmias, neurological disorders, and other diseases, highlighting its importance in cellular function and signaling pathways. Recombinant KCNJ4 protein expression systems have been developed to study its biophysical properties, pharmacological profiles, and interactions with other cellular proteins. Understanding the structure-function relationship of KCNJ4 through recombinant protein studies can provide insights into the mechanisms underlying its role in health and disease, paving the way for the development of novel therapeutic strategies targeting potassium channels to treat arrhythmias and other related conditions. Additionally, KCNJ4’s role in drug interactions and its potential as a biomarker for certain diseases further emphasizes the necessity for continued research in this area to explore its full therapeutic potential.
