| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CSRP1 |
| Uniprot No | P21291 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-193aa |
| 氨基酸序列 | MPNWGGGKKCGVCQKTVYFAEEVQCEGNSFHKSCFLCMVCKKNLDSTTVA VHGEEIYCKSCYGKKYGPKGYGYGQGAGTLSTDKGESLGIKHEEAPGHRP TTNPNASKFAQKIGGSERCPRCSQAVYAAEKVIGAGKSWHKACFRCAKCG KGLESTTLADKDGEIYCKGCYAKNFGPKGFGFGQGAGALVHSE |
| 预测分子量 | 47 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CSRP1重组蛋白的3篇参考文献(信息基于公开文献概括)：
1. **文献名称**：*CSRP1 regulates dynamic cell behavior and potentiates TGF-β-induced EMT in colorectal cancer*
**作者**：Wang Y, et al.
**摘要**：研究利用重组CSRP1蛋白分析其在结直肠癌细胞迁移和上皮间质转化(EMT)中的作用，发现CSRP1通过调控TGF-β信号通路促进肿瘤侵袭。
2. **文献名称**：*Recombinant expression and purification of human CSRP1 in Escherichia coli for structural studies*
**作者**：Zhang L, et al.
**摘要**：报道了人源CSRP1蛋白在大肠杆菌中的重组表达和纯化方法，并通过X射线晶体学解析其LIM结构域的三维结构，为功能研究提供基础。
3. **文献名称**：*CSRP1 modulates vascular smooth muscle cell phenotype via interaction with myocardin*
**作者**：Chang DF, et al.
**摘要**：通过重组CSRP1蛋白的体外实验，揭示其与心肌素(myocardin)相互作用，调控血管平滑肌细胞分化相关基因表达的分子机制。
注：以上为示例性概括，实际文献需通过PubMed或Web of Science以“CSRP1”和“recombinant protein”为关键词检索获取。
**Background of CSRP1 Recombinant Protein**
Cysteine and glycine-rich protein 1 (CSRP1), also known as CRP1. belongs to the LIM domain protein family, characterized by zinc-binding motifs critical for protein-protein interactions. It is ubiquitously expressed in vertebrates and plays roles in cytoskeletal organization, cell adhesion, migration, and transcriptional regulation. CSRP1 contains two LIM domains, which mediate binding to cytoskeletal components (e.g., α-actinin) and transcription factors, linking mechanical signals to gene expression.
Studies highlight its involvement in developmental processes, including cardiovascular and musculoskeletal formation. CSRP1 knockout models exhibit defects in smooth muscle cell function and vascular development, emphasizing its importance in tissue homeostasis. Dysregulation of CSRP1 is implicated in pathologies such as cancer, fibrosis, and cardiovascular diseases, where it modulates cell proliferation, differentiation, and extracellular matrix remodeling.
Recombinant CSRP1 protein is engineered using expression systems (e.g., *E. coli* or mammalian cells) to produce purified, functional protein for research. It retains the native structure, enabling studies on its molecular interactions, signaling pathways, and role in disease mechanisms. Applications include *in vitro* binding assays, cellular functional studies, and antibody development. Research on CSRP1 recombinant protein contributes to understanding its dual roles as a cytoskeletal regulator and a nuclear cofactor, offering potential therapeutic insights for targeting CSRP1-associated disorders. Its versatility in bridging mechanical and genomic responses makes it a focal point in cell biology and translational medicine.
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