Rabbit Polyclonal PARD3 Antibody

The PARD3 antibody is a crucial tool for studying the PAR-3 family member, partitioning defective 3 homolog (PARD3), a scaffold protein essential for establishing and maintaining cell polarity. PARD3. part of the conserved PAR complex (with PARD6 and atypical protein kinase C), orchestrates asymmetric cell division, epithelial tight junction formation, and neuronal polarization. It contains PDZ domains, a CR1 region, and an aPKC-binding motif, enabling interactions with signaling proteins, cytoskeletal components, and junctional complexes. Dysregulation of PARD3 is linked to cancer progression, neurodevelopmental disorders, and tissue morphogenesis defects. For instance, reduced PARD3 expression in tumors correlates with metastasis and poor prognosis, while mutations disrupt neuronal connectivity. Researchers use PARD3 antibodies in techniques like Western blotting, immunofluorescence, and immunohistochemistry to visualize its localization (e.g., cell-cell junctions, apical membranes) and quantify expression levels in disease models. These studies help unravel mechanisms underlying polarity-related pathologies and potential therapeutic targets. Commercial PARD3 antibodies are typically validated for specificity against human, mouse, or rat isoforms, with careful optimization required for experimental conditions to avoid cross-reactivity.