| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TAPBP |
| Uniprot No | O15533 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 197-300aa |
| 氨基酸序列 | PGPPPFGLEWRRQHLGKGHLLLAATPGLNGQMPAAQEGAVAFAAWDDDEP WGPWTGNGTFWLPTVQPFQEGTYLATIHLPYLQGQVTLELAVYKPPKVSL MPAT |
| 预测分子量 | 37 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TAPBP重组蛋白的参考文献示例(注：内容为示例格式，实际文献请通过学术数据库查询)：
1. **文献名称**: "Structural and functional analysis of the TAPBP protein in antigen processing"
**作者**: Abele, R., Tampé, R.
**摘要**: 研究利用重组TAPBP蛋白解析其与抗原肽转运复合体(TAP)的相互作用，揭示其在MHC I类分子抗原呈递中的关键作用，并通过体外重组表达验证其稳定性。
2. **文献名称**: "Recombinant TAPBP enhances MHC I surface expression in tumor cells"
**作者**: Chen, L., et al.
**摘要**: 通过表达重组TAPBP蛋白，证明其能恢复肿瘤细胞中MHC I分子的表面表达，为免疫逃逸机制提供新的治疗靶点。
3. **文献名称**: "Expression and purification of human TAPBP in E. coli for biochemical studies"
**作者**: Koch, J., et al.
**摘要**: 描述在大肠杆菌系统中高效表达和纯化功能性重组TAPBP蛋白的方法，并评估其在体外结合TAP复合体的活性。
4. **文献名称**: "TAPBP as a modulator of viral immune evasion: Insights from recombinant protein assays"
**作者**: Neumann, L., et al.
**摘要**: 利用重组TAPBP蛋白研究病毒蛋白如何干扰抗原呈递通路，发现其与病毒蛋白的竞争性结合机制。
建议通过 **PubMed/Google Scholar** 搜索关键词 "TAPBP recombinant protein" 或结合具体研究领域筛选最新文献。
TAPBP (Transporter Associated with Antigen Processing Binding Protein), also known as tapasin, is a critical component of the major histocompatibility complex (MHC) class I antigen presentation pathway. It plays a central role in adaptive immunity by facilitating the loading of antigenic peptides onto MHC class I molecules, which are then displayed on the cell surface for recognition by CD8+ T cells. This process is essential for immune surveillance against intracellular pathogens and malignant cells. Structurally, TAPBP is a transmembrane glycoprotein localized to the endoplasmic reticulum (ER), where it forms a bridge between the peptide-transporting TAP complex (TAP1/TAP2) and MHC class I molecules, stabilizing their interaction and enhancing peptide selection efficiency.
Recombinant TAPBP refers to the protein produced in vitro using genetic engineering techniques, often expressed in mammalian, insect, or bacterial systems. Its production enables detailed biochemical and functional studies, including structural analysis of the MHC class I peptide-loading complex and exploration of immune evasion mechanisms employed by viruses or cancers. Dysregulation of TAPBP has been implicated in autoimmune diseases and cancer progression, making it a potential therapeutic target. Recombinant forms are also utilized in immunotherapy research, such as optimizing antigen presentation in dendritic cell vaccines. By studying TAPBP, researchers aim to refine strategies for enhancing immune responses in vaccines or overcoming immune escape in malignancies.
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