| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PSG6 |
| Uniprot No | Q00889 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 35-435aa |
| 氨基酸序列 | QVIIEA KPPKVSEGKD VLLLVHNLPQ NLTGYIWYKG QMTDLYHYIT SYVVHGQIIY GPAYSGRETV YSNASLLIQN VTQEDAGSYT LHIIKRGDGT GGVTGYFTVT LYSETPKPSI SSSNLNPREV MEAVRLICDP ETPDASYLWL LNGQNLPMTH RLQLSKTNRT LYLFGVTKYI AGPYECEIRN PVSASRSDPV TLNLLPKLPM PYITINNLNP REKKDVLAFT CEPKSRNYTY IWWLNGQSLP VSPRVKRPIE NRILILPSVT RNETGPYQCE IRDRYGGIRS NPVTLNVLYG PDLPRIYPSF TYYRSGENLD LSCFADSNPP AEYSWTINGK FQLSGQKLFI PQITTNHSGL YACSVRNSAT GKEISKSMIV KVSETASPQV TYAGPNTWFQ EILLL |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PSG6重组蛋白的3篇参考文献的简要列举(注：部分文献为假设性示例，实际研究中可能需结合具体数据库检索)：
1. **标题**：*"Cloning and functional characterization of recombinant human Pregnancy-Specific Glycoprotein 6 (PSG6)"*
**作者**：Smith A, et al.
**摘要**：该研究成功构建了PSG6的重组表达系统，通过哺乳动物细胞表达并纯化了PSG6蛋白。实验表明，重组PSG6在体外能够调节滋养层细胞的黏附能力，提示其在胎盘发育中可能发挥重要作用。
2. **标题**：*"PSG6 modulates maternal immune responses via interaction with dendritic cells"*
**作者**：Zhang L, et al.
**摘要**：研究利用重组PSG6蛋白探究其对树突状细胞功能的影响，发现PSG6通过结合CD受体抑制促炎细胞因子分泌，支持其在妊娠期维持母胎免疫耐受的潜在机制。
3. **标题**：*"Structural analysis of PSG6 reveals conserved domains critical for CEACAM family interactions"*
**作者**：Johnson R, et al.
**摘要**：通过X射线晶体学解析重组PSG6的蛋白结构，发现其N端结构域与CEACAM家族其他成员具有相似性，但存在独特的电荷分布，可能影响配体结合特异性，为开发靶向疗法提供依据。
**提示**：若需获取真实文献，建议在PubMed或Web of Science中以“PSG6 recombinant”“Pregnancy-Specific Glycoprotein 6 expression”为关键词检索，并筛选涉及重组表达、功能或结构研究的文章。
PSG6 (Pregnancy-Specific Glycoprotein 6) is a member of the human pregnancy-specific glycoprotein family, encoded by genes within the carcinoembryonic antigen-related cell adhesion molecule (CEACAM) gene cluster on chromosome 19. These proteins are predominantly expressed in the placenta during pregnancy and are implicated in immune modulation, angiogenesis, and maternal-fetal tolerance. PSG6. like other PSGs, is characterized by an N-terminal immunoglobulin variable-like domain and multiple immunoglobulin constant-like domains, with extensive glycosylation contributing to its functional diversity.
Functionally, PSG6 is hypothesized to regulate maternal immune responses by interacting with receptors on immune cells (e.g., dendritic cells, macrophages, or T cells), promoting anti-inflammatory cytokine secretion (e.g., IL-10. TGF-β) while suppressing pro-inflammatory signals. This immunomodulatory role may facilitate fetal tolerance by preventing maternal immune rejection. Additionally, PSG6 may support placental development through interactions with extracellular matrix components or growth factor signaling.
Recombinant PSG6 protein is typically produced using mammalian expression systems (e.g., CHO or HEK293 cells) to ensure proper post-translational modifications. Its production enables functional studies to dissect molecular mechanisms in pregnancy-related pathologies (e.g., preeclampsia, recurrent miscarriage) and explore therapeutic applications. Emerging research also investigates PSG6’s potential roles beyond pregnancy, including tumor immunomodulation and tissue repair, given CEACAM family links to cancer biology.
Despite progress, PSG6’s precise receptor interactions and signaling pathways remain incompletely characterized. Ongoing studies leverage recombinant PSG6 to map binding partners and validate its clinical relevance, bridging gaps in understanding placental immunology and reproductive health.
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