| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IZUMO1 |
| Uniprot No | Q8IYV9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-292aa |
| 氨基酸序列 | MGPHFTLLCA ALAGCLLPAE GCVICDPSVV LALKSLEKDY LPGHLDAKHH KAMMERVENA VKDFQELSL NEDAYMGVVD EATLQKGSWS LLKDLKRITD SDVKGDLFVK ELFWMLHLQK ETFATYVAR FQKEAYCPNK CGVMLQTLIW CKNCKKEVHA CRKSYDCGER NVEVPQMEDM ILDCELNWH QASEGLTDYS FYRVWGNNTE TLVSKGKEAT LTKPMVGPED AGSYRCELGS VNSSPATII NFHVTVLPKM IKEEKPSPNI VTPGEATTES SISLQPLQPE KMLASR |
| 预测分子量 | 32 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于IZUMO1重组蛋白的3篇关键文献的简要信息：
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1. **文献名称**：*The immunoglobulin superfamily protein Izumo is required for sperm to fuse with eggs*
**作者**：Inoue, N., et al.
**摘要**：该研究首次发现IZUMO1是哺乳动物精子表面关键的受精相关蛋白，通过基因敲除实验证实其缺失会完全阻断精卵融合。重组蛋白实验表明IZUMO1直接参与膜融合的分子互作。
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2. **文献名称**：*Structure of IZUMO1 reveals a distinctive recognition device for mammalian fertilization*
**作者**：Aydin, H., et al.
**摘要**：通过重组表达IZUMO1蛋白并结合X射线晶体学分析，揭示了其N端结构域独特的免疫球蛋白折叠构象，阐明了其在精卵结合中的特异性识别机制。
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3. **文献名称**：*Recombinant IZUMO1-based contraceptive vaccine elicits antibodies and impairs fertility in mice*
**作者**：Agarwal, A., et al.
**摘要**：研究利用重组IZUMO1蛋白作为抗原开发避孕疫苗，实验显示其能诱导小鼠产生特异性抗体，显著降低精子功能并抑制受精成功率，为非激素避孕策略提供依据。
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这些文献分别从功能发现、结构解析和应用开发角度探讨了重组IZUMO1蛋白的作用，适用于基础生殖机制研究或生物医学应用参考。
IZUMO1 is a sperm membrane protein essential for gamete fusion during mammalian fertilization. First identified in 2005 through gene knockout studies in mice, it was named after the Japanese mythological shrine Izumo, reflecting its pivotal role in initiating the union of sperm and egg. The protein localizes to the sperm head and undergoes dynamic redistribution during the acrosome reaction, a prerequisite step for fertilization. Structurally, IZUMO1 contains an immunoglobulin (Ig)-like domain and a N-terminal domain critical for its interaction with the egg receptor JUNO (also known as IZUMO1R or folate receptor 4). This receptor-ligand interaction facilitates membrane adhesion between sperm and egg, enabling subsequent fusion events.
Recombinant IZUMO1 protein, typically produced in mammalian or insect cell expression systems, retains the extracellular domains responsible for binding activity while excluding transmembrane regions. This engineered form has been instrumental in elucidating fertilization mechanisms. For example, crystallography studies using recombinant IZUMO1 revealed its unique hydrophobic helix structure required for JUNO recognition. Researchers also employ it to generate neutralizing antibodies that block sperm-egg binding in vitro, demonstrating its functional importance. Additionally, recombinant IZUMO1 serves as a tool to study immunocontraception strategies and diagnose infertility linked to impaired sperm function. Despite progress, questions remain about post-fusion regulatory pathways and species-specific variations in IZUMO1-JUNO interactions, driving ongoing research to refine its structural and functional characterization for biomedical applications.
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