| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ETRB |
| Uniprot No | P24530 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 27-442aa |
| 氨基酸序列 | EERGFPPDRATPLLQTAEIMTPPTKTLWPKGSNASLARSLAPAEVPKGDRTAGSPPRTISPPPCQGPIEIKETFKYINTVVSCLVFVLGIIGNSTLLRIIYKNKCMRNGPNILIASLALGDLLHIVIDIPINVYKLLAEDWPFGAEMCKLVPFIQKASVGITVLSLCALSIDRYRAVASWSRIKGIGVPKWTAVEIVLIWVVSVVLAVPEAIGFDIITMDYKGSYLRICLLHPVQKTAFMQFYKTAKDWWLFSFYFCLPLAITAFFYTLMTCEMLRKKSGMQIALNDHLKQRREVAKTVFCLVLVFALCWLPLHLSRILKLTLYNQNDPNRCELLSFLLVLDYIGINMASLNSCINPIALYLVSKRFKNCFKSCLCCWCQSFEEKQSLEEKQSCLKFKANDHGYDNFRSSNKYSSS |
| 预测分子量 | 50.3kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ETRB(可能为Endothelin Receptor Type B，即ETB受体)重组蛋白研究的3篇代表性文献，供参考：
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1. **文献名称**：Cloning and expression of a cDNA encoding an endothelin receptor
**作者**：Hosoda K, et al.
**摘要**：该研究首次报道了人内皮素B型受体(ETB)的cDNA克隆及重组表达，分析了其在细胞膜上的信号转导功能，为后续研究ETB的生理病理机制奠定了基础。发表于*PNAS* (1992)。
2. **文献名称**：Endothelin receptor subtypes and their role in transmembrane signaling mechanisms
**作者**：Yanagisawa M, et al.
**摘要**：综述了ETB受体与其他亚型(如ETA)的结构差异，探讨重组ETB蛋白在细胞内的G蛋白偶联信号通路中的作用，及其在血管收缩和细胞增殖中的功能。发表于*Pharmacological Reviews* (1998)。
3. **文献名称**：Recombinant endothelin-B receptor ligand-binding domain: production, purification, and structural characterization
**作者**：Okamoto Y, Moriyama T
**摘要**：通过大肠杆菌系统表达并纯化ETB受体的配体结合结构域重组蛋白，结合X射线晶体学解析其三维结构，揭示了内皮素分子与受体结合的关键位点。发表于*Journal of Biological Chemistry* (2005)。
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**注**：若"ETRB"为其他蛋白缩写，建议结合具体研究领域(如植物学中的ETRB蛋白)进一步修正检索关键词。
The ETRB (Endothelin Receptor Type B) recombinant protein is a key component in studying the endothelin signaling pathway, which plays critical roles in cardiovascular regulation, cell proliferation, and tissue homeostasis. Endothelin receptors, classified as ETA and ETB, are G protein-coupled receptors (GPCRs) that bind endothelin peptides (ET-1. ET-2. ET-3). Specifically, ETBR is primarily expressed in endothelial cells, smooth muscle, and the nervous system. Unlike ETA, which predominantly mediates vasoconstriction, ETBR often counterbalances these effects by promoting vasodilation through nitric oxide release and clearing endothelin via receptor-mediated internalization.
Recombinant ETBR proteins are engineered using molecular cloning techniques, typically expressed in mammalian systems (e.g., HEK293 or CHO cells) to ensure proper post-translational modifications and membrane localization. These proteins retain functional domains, including seven transmembrane helices and ligand-binding sites, enabling in vitro studies of receptor-ligand interactions, signal transduction mechanisms (e.g., Gαi/o and Gαq coupling), and downstream effects like MAPK activation or calcium mobilization.
ETBR dysregulation is implicated in pathologies such as pulmonary hypertension, cancer metastasis, and neurodegenerative disorders. Recombinant ETBR serves as a vital tool for drug discovery, aiding in the development of antagonists (e.g., bosentan) or agonists targeting endothelin-related diseases. It also facilitates structural studies (e.g., X-ray crystallography) to elucidate receptor activation mechanisms and design selective therapeutics. Current research focuses on ETBR's dual role in pro- and anti-tumorigenic signaling, highlighting its therapeutic potential in precision medicine. Challenges remain in optimizing receptor stability for biophysical assays and addressing species-specific ligand affinity differences during preclinical testing.
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