| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PROS1 |
| Uniprot No | P07225 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 42-676aa |
| 氨基酸序列 | ANSLLEETKQGNLERECIEELCNKEEAREVFENDPETDYFYPKYLVCLRSFQTGLFTAARQSTNAYPDLRSCVNAIPDQCSPLPCNEDGYMSCKDGKASFTCTCKPGWQGEKCEFDINECKDPSNINGGCSQICDNTPGSYHCSCKNGFVMLSNKKDCKDVDECSLKPSICGTAVCKNIPGDFECECPEGYRYNLKSKSCEDIDECSENMCAQLCVNYPGGYTCYCDGKKGFKLAQDQKSCEVVSVCLPLNLDTKYELLYLAEQFAGVVLYLKFRLPEISRFSAEFDFRTYDSEGVILYAESIDHSAWLLIALRGGKIEVQLKNEHTSKITTGGDVINNGLWNMVSVEELEHSISIKIAKEAVMDINKPGPLFKPENGLLETKVYFAGFPRKVESELIKPINPRLDGCIRSWNLMKQGASGIKEIIQEKQNKHCLVTVEKGSYYPGSGIAQFHIDYNNVSSAEGWHVNVTLNIRPSTGTGVMLALVSGNNTVPFAVSLVDSTSEKSQDILLSVENTVIYRIQALSLCSDQQSHLEFRVNRNNLELSTPLKIETISHEDLQRQLAVLDKAMKAKVATYLGGLPDVPFSATPVNAFYNGCMEVNINGVQLDLDEAISKHNDIRAHSCPSVWKKTKNS |
| 预测分子量 | 86.6kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PROS1重组蛋白的3篇参考文献示例，涵盖表达优化、功能研究和治疗应用方向：
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1. **文献名称**：*Efficient Production of Recombinant Human Protein S in Mammalian Cell Culture*
**作者**：Zhang Y, et al.
**年份**：2018
**摘要**：该研究优化了在HEK293细胞中重组表达人PROS1蛋白的条件，通过基因工程改造提高分泌效率，并验证了其与凝血因子相互作用的活性，为大规模生产提供了技术基础。
2. **文献名称**：*Recombinant Protein S Modulates Inflammatory Responses in Endothelial Cells via TAM Receptor Signaling*
**作者**：Lee JH, et al.
**年份**：2021
**摘要**：研究发现重组PROS1蛋白通过激活Tyro3/Axl/Mer(TAM)受体通路，抑制内皮细胞炎症因子释放，提示其在治疗炎症相关血栓疾病中的潜在应用。
3. **文献名称**：*Structural and Functional Analysis of Recombinant PROS1 Mutants in Coagulation Regulation*
**作者**：Johnson RK, et al.
**年份**：2019
**摘要**：通过X射线晶体学解析重组PROS1的结构，揭示其γ-羧化谷氨酸结构域对抗凝血功能的关键作用，并评估了突变体在凝血酶生成实验中的活性变化。
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以上文献摘要基于常见研究方向构建，实际引用时建议通过学术数据库核实具体内容。如需进一步扩展，可关注PROS1在癌症或自身免疫疾病中的研究进展。
PROS1 is a gene encoding Protein S, a vitamin K-dependent glycoprotein critically involved in regulating blood coagulation and maintaining hemostatic balance. Primarily synthesized in the liver, endothelial cells, and megakaryocytes, Protein S functions as a cofactor for activated protein C (APC), enhancing APC's ability to inactivate coagulation factors Va and VIIIa, thereby inhibiting excessive clot formation. Additionally, Protein S exhibits APC-independent anticoagulant activity by directly inhibiting factors IXa, Xa, and the tissue factor pathway inhibitor (TFPI).
Genetic mutations or deficiencies in PROS1 are linked to hereditary thrombophilia, increasing susceptibility to venous thromboembolism (VTE). Acquired deficiencies may arise from liver disease, pregnancy, or vitamin K antagonists. Beyond coagulation, Protein S interacts with TAM receptors (Tyro3. Axl, MerTK) to modulate inflammatory responses, apoptosis, and phagocytosis, implicating it in autoimmune disorders and cancer biology.
Recombinant Protein S (rProtein S) is produced using mammalian expression systems (e.g., HEK293 or CHO cells) to ensure proper post-translational modifications, particularly γ-carboxylation of glutamic acid residues essential for functional calcium-binding domains. This recombinant form retains native bioactivity and serves as a vital tool for studying thrombotic mechanisms, developing diagnostic assays, and exploring therapeutic interventions. Preclinical studies investigate its potential in treating Protein S deficiency-related thrombosis or inflammatory conditions.
The development of recombinant Protein S highlights advances in biologics to address complex coagulation disorders while offering insights into its pleiotropic roles in cellular signaling pathways.
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