| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HSPD1 / HSP60 |
| Uniprot No | P10809 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 27-573aa |
| 氨基酸序列 | AKDV KFGADARALM LQGVDLLADA VAVTMGPKGR TVIIEQSWGS PKVTKDGVTV AKSIDLKDKY KNIGAKLVQD VANNTNEEAG DGTTTATVLA RSIAKEGFEK ISKGANPVEI RRGVMLAVDA VIAELKKQSK PVTTPEEIAQ VATISANGDK EIGNIISDAM KKVGRKGVIT VKDGKTLNDE LEIIEGMKFD RGYISPYFIN TSKGQKCEFQ DAYVLLSEKK ISSIQSIVPA LEIANAHRKP LVIIAEDVDG EALSTLVLNR LKVGLQVVAV KAPGFGDNRK NQLKDMAIAT GGAVFGEEGL TLNLEDVQPH DLGKVGEVIV TKDDAMLLKG KGDKAQIEKR IQEIIEQLDV TTSEYEKEKL NERLAKLSDG VAVLKVGGTS DVEVNEKKDR VTDALNATRA AVEEGIVLGG GCALLRCIPA LDSLTPANED QKIGIEIIKR TLKIPAMTIA KNAGVEGSLI VEKIMQSSSE VGYDAMAGDF VNMVEKGIID PTKVVRTALL DAAGVASLLT TAEVVVTEIP KEEKDPGMGA MGGMGGGMGG GMF |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Recombinant human HSP60 produced in Escherichia coli enhances antigen-specific T-cell responses in vitro"**
- **作者**: Zanin-Zhorov, A. et al.
- **摘要**: 研究通过大肠杆菌表达重组人HSP60蛋白，验证其分子伴侣活性及免疫调节功能，发现其可增强抗原特异性T细胞反应，提示在免疫治疗中的应用潜力。
2. **"Production and characterization of recombinant HSP60 (GroEL) from Chlamydia pneumoniae"**
- **作者**: Kol, A. et al.
- **摘要**: 报道肺炎衣原体HSP60(GroEL同源物)的重组表达与纯化，分析其促炎特性，揭示其在动脉粥样硬化等慢性炎症疾病中的潜在作用机制。
3. **"Functional analysis of mitochondrial HSP60 chaperonin in Parkinson’s disease models"**
- **作者**: De Mena, L. et al.
- **摘要**: 利用重组HSP60蛋白研究其在帕金森病模型中对线粒体蛋白错误折叠的修复能力，表明HSP60过表达可减轻神经细胞毒性，为神经退行性疾病提供治疗靶点。
4. **"Recombinant HSP60 from Helicobacter pylori induces pro-inflammatory cytokine production in macrophages"**
- **作者**: Takeda, K. et al.
- **摘要**: 通过重组幽门螺杆菌HSP60验证其激活巨噬细胞并诱导IL-6、TNF-α等炎症因子释放的作用，探讨其在胃部感染及免疫病理中的角色。
HSPD1. also known as heat shock protein 60 (HSP60), is a mitochondrial chaperonin encoded by the HSPD1 gene in humans. As a member of the heat shock protein family, it plays a critical role in maintaining cellular homeostasis by assisting in the folding and assembly of newly synthesized proteins within mitochondria. HSP60 forms a characteristic double-ring structure that binds unfolded polypeptides in an ATP-dependent manner, preventing misfolding and aggregation under stress conditions. Its expression is upregulated during cellular stress, such as heat shock, oxidative damage, or pathogen invasion, to protect mitochondrial integrity.
Recombinant HSP60 proteins are engineered using expression systems like *E. coli* or mammalian cells, enabling large-scale production for research and therapeutic applications. These proteins retain the functional domains required for chaperone activity, making them valuable tools for studying mitochondrial protein dynamics, stress response pathways, and disease mechanisms. Dysregulation of HSP60 has been linked to neurodegenerative disorders (e.g., Alzheimer’s), autoimmune diseases (e.g., rheumatoid arthritis), and cancer, where it may promote tumor survival by inhibiting apoptosis.
Notably, HSP60 exhibits dual roles in immunity. While it assists in antigen presentation and modulates inflammatory responses, its extracellular release can trigger autoimmune reactions through molecular mimicry. Recombinant HSP60 is being explored as a diagnostic biomarker for inflammatory conditions and a therapeutic target. In vaccine development, its immunomodulatory properties are harnessed to enhance immune responses against pathogens or tumor antigens. Ongoing research also investigates its potential in mitochondrial-targeted therapies and as a stress-indicator in aging-related studies. However, its context-dependent pro-inflammatory or anti-inflammatory effects require careful evaluation in clinical applications.
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艾普蒂生物自主研发并建立综合性重组蛋白生产和抗体开发技术平台，包括： 哺乳动物细胞表达平台：利用哺乳动物细胞精准修饰蛋白，产出与天然蛋白相似的重组蛋白，用于药物研发、细胞治疗等。 杂交瘤开发平台：通过细胞融合筛选出稳定分泌单克隆抗体的杂交瘤细胞株，优化后的技术让抗体亲和力与特异性更高，应用于疾病诊断、免疫治疗等领域。 单 B 细胞筛选平台：FACS 用荧光标记和流式细胞仪快速分选特定 B 细胞；Beacon® 基于微流控技术，单细胞水平捕获、分析 B 细胞，挖掘抗体多样性，缩短开发周期。 凭借这些平台，艾普蒂生物为客户提供优质试剂和专业 CRO 技术服务，推动生物科技发展。
艾普蒂生物在重组蛋白和天然蛋白开发领域经验十分丰富，拥有超过 2 万种重组蛋白的开发案例。在四大重组蛋白表达平台的运用上，艾普蒂生物不仅经验老到，还积累了详实的成功案例。针对客户的工业化生产需求，我们能够定制并优化实验方案。通过小试探索、工艺放大以及条件优化等环节，对重组蛋白基因序列进行优化，全面探索多种条件，精准找出最契合客户需求的生产方法。 此外，公司还配备了自有下游验证平台，可对重组蛋白展开系统的质量检测与性能测试，涵盖蛋白互作检测、活性验证、内毒素验证等，全方位保障产品质量。 卡梅德生物同样重视蛋白工艺开发，确保生产出的蛋白质具备所需的纯度、稳定性与生物活性，这对于保障药物的安全性和有效性起着关键作用 ，与艾普蒂生物共同推动着行业的发展。
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