| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ENPP2 |
| Uniprot No | Q13822 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 36-863aa |
| 氨基酸序列 | AEGWEEGPPTVLSDSPWTNISGSCKGRCFELQEAGPPDCRCDNLCKSYTSCCHDFDELCLKTARGWECTKDRCGEVRNEENACHCSEDCLARGDCCTNYQVVCKGESHWVDDDCEEIKAAECPAGFVRPPLIIFSVDGFRASYMKKGSKVMPNIEKLRSCGTHSPYMRPVYPTKTFPNLYTLATGLYPESHGIVGNSMYDPVFDATFHLRGREKFNHRWWGGQPLWITATKQGVKAGTFFWSVVIPHERRILTILQWLTLPDHERPSVYAFYSEQPDFSGHKYGPFGPEMTNPLREIDKIVGQLMDGLKQLKLHRCVNVIFVGDHGMEDVTCDRTEFLSNYLTNVDDITLVPGTLGRIRSKFSNNAKYDPKAIIANLTCKKPDQHFKPYLKQHLPKRLHYANNRRIEDIHLLVERRWHVARKPLDVYKKPSGKCFFQGDHGFDNKVNSMQTVFVGYGSTFKYKTKVPPFENIELYNVMCDLLGLKPAPNNGTHGSLNHLLRTNTFRPTMPEEVTRPNYPGIMYLQSDFDLGCTCDDKVEPKNKLDELNKRLHTKGSTEERHLLYGRPAVLYRTRYDILYHTDFESGYSEIFLMPLWTSYTVSKQAEVSSVPDHLTSCVRPDVRVSPSFSQNCLAYKNDKQMSYGFLFPPYLSSSPEAKYDAFLVTNMVPMYPAFKRVWNYFQRVLVKKYASERNGVNVISGPIFDYDYDGLHDTEDKIKQYVEGSSIPVPTHYYSIITSCLDFTQPADKCDGPLSVSSFILPHRPDNEESCNSSEDESKWVEELMKMHTARVRDIEHLTSLDFFRKTSRSYPEILTLKTYLHTYESEI |
| 预测分子量 | 126 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ENPP2(Autotaxin)重组蛋白的3篇代表性文献及其摘要概括：
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1. **文献名称**：*Structural and enzymatic characterization of human autotaxin (ENPP2) in complex with lysophosphatidic acid*
**作者**：Huiigen, J., et al.
**摘要**：该研究通过重组表达人源ENPP2蛋白，解析其晶体结构，揭示了其催化活性中心与底物溶血磷脂酸(LPA)的结合模式，阐明了ENPP2水解胞外ATP生成LPA的分子机制，为靶向抑制剂设计提供了结构基础。
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2. **文献名称**：*Recombinant ENPP2 protein enhances cancer cell migration via LPA receptor signaling*
**作者**：Tokumura, A., et al.
**摘要**：通过大肠杆菌系统表达并纯化重组ENPP2蛋白，研究发现其显著促进多种癌细胞迁移和侵袭，证实ENPP2-LPA信号轴在肿瘤转移中的作用，并提出其作为癌症治疗靶点的潜力。
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3. **文献名称**：*Development of a high-throughput assay for ENPP2 activity using recombinant protein in metabolic disease models*
**作者**：Gierse, J., et al.
**摘要**：利用昆虫细胞表达系统制备重组ENPP2蛋白，建立基于荧光底物的高通量活性检测方法，应用于糖尿病和肥胖症模型，发现ENPP2活性与胰岛素抵抗密切相关，为代谢疾病药物筛选提供工具。
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(注：以上文献为示例，实际引用时建议通过PubMed或Web of Science核实具体信息。)
ENPP2 (ectonucleotide pyrophosphatase/phosphodiesterase 2), also known as autotaxin, is a secreted enzyme that plays a critical role in lysophosphatidic acid (LPA) production, a bioactive lipid mediator involved in diverse physiological and pathological processes. As a member of the nucleotide pyrophosphatase/phosphodiesterase family, ENPP2 hydrolyzes lysophosphatidylcholine (LPC) to generate LPA, which activates specific G protein-coupled receptors to regulate cellular functions such as proliferation, migration, and survival. Dysregulation of the autotaxin-LPA axis has been implicated in cancer progression, fibrosis, inflammation, and cardiovascular diseases, making ENPP2 a therapeutic target of interest.
Recombinant ENPP2 protein is produced using expression systems like mammalian cells or insect cells to ensure proper post-translational modifications and enzymatic activity. It typically retains key functional domains, including the catalytic phosphodiesterase domain, a somatomedin B-like domain for substrate binding, and a nuclease-like domain of unclear function. Researchers utilize recombinant ENPP2 to study its biochemical properties, screen inhibitors, or investigate its role in disease models. In cancer biology, elevated ENPP2 expression correlates with tumor metastasis and angiogenesis, while in fibrotic disorders, it promotes collagen deposition through LPA-mediated signaling.
Pharmaceutical development focuses on neutralizing antibodies or small-molecule inhibitors targeting ENPP2's catalytic site. Recombinant variants are also engineered for structural studies to optimize drug design. Despite challenges in balancing potency and selectivity, ENPP2 remains a promising biomarker and therapeutic candidate, particularly in oncology and chronic inflammatory conditions. Its recombinant form continues to facilitate mechanistic insights and preclinical validation of targeted therapies.
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