| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | AFP |
| Uniprot No | Q12899 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-609aa |
| 氨基酸序列 | MKWVESIFLIFLLNFTESRTLHRNEYGIASILDSYQCTAEISLADLATIFFAQFVQEATYKEVSKMVKDALTAIEKPTGDEQSSGCLENQLPAFLEELCHEKEILEKYGHSDCCSQSEEGRHNCFLAHKKPTPASIPLFQVPEPVTSCEAYEEDRETFMNKFIYEIARRHPFLYAPTILLWAARYDKIIPSCCKAENAVECFQTKAATVTKELRESSLLNQHACAVMKNFGTRTFQAITVTKLSQKFTKVNFTEIQKLVLDVAHVHEHCCRGDVLDCLQDGEKIMSYICSQQDTLSNKITECCKLTTLERGQCIIHAENDEKPEGLSPNLNRFLGDRDFNQFSSGEKNIFLASFVHEYSRRHPQLAVSVILRVAKGYQELLEKCFQTENPLECQDKGEEELQKYIQESQALAKRSCGLFQKLGEYYLQNAFLVAYTKKAPQLTSSELMAITRKMAATAATCCQLSEDKLLACGEGAADIIIGHLCIRHEMTPVNTGVGQCCTSSYANRRPCFSSLVVDETYVPPAFSDDKFIFHKDLCQAQGVALQTMKQEFLINLVKQKPQITEEQLEAVIADFSGLLEKCCQGQEQEVCFAEEGQKLISKTRAALGV |
| 分子量 | 95.1 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为假设性参考文献示例(实际文献可能需要检索PubMed等数据库验证)：
1. **"TRIM26 negatively regulates interferon-β production and antiviral response through targeting RIG-I"**
Wang et al., Cellular Immunology (2020).
**摘要**：该研究揭示TRIM26作为E3泛素连接酶，通过介导RIG-I的泛素化修饰抑制其活性，从而负调控I型干扰素信号通路和抗病毒天然免疫应答。
2. **"TRIM26 promotes hepatocellular carcinoma progression by enhancing Wnt/β-catenin signaling"**
Zhang et al., Oncogene (2019).
**摘要**：研究表明TRIM26在肝细胞癌中高表达，通过稳定β-catenin蛋白促进肿瘤细胞增殖和转移，提示其作为潜在治疗靶点。
3. **"TRIM26 modulates autophagic flux by interacting with LC3 in neurodegenerative diseases"**
Li et al., Autophagy (2021).
**摘要**：作者发现TRIM26通过与自噬标记蛋白LC3相互作用，调控自噬体成熟，影响神经细胞中异常蛋白聚集的清除，与阿尔茨海默病相关。
4. **"TRIM26 suppresses HIV-1 replication by activating type I IFN pathway"**
Suzuki et al., Journal of Virology (2018).
**摘要**：研究证明TRIM26通过促进干扰素刺激基因(ISGs)表达，抑制HIV-1病毒在宿主细胞内的复制，揭示其抗逆转录病毒机制。
**注意**：以上为基于TRIM26已知功能的示例，真实文献需查阅学术数据库确认。如需具体文献，建议通过PubMed搜索关键词(如TRIM26 immune/cancer/virus)。
TRIM26. a member of the tripartite motif (TRIM) protein family, is characterized by its conserved N-terminal domains: a RING finger, B-box, and coiled-coil region, which mediate ubiquitination, protein interactions, and subcellular localization. Primarily localized in the nucleus, TRIM26 plays multifaceted roles in innate immunity, inflammation, and cancer progression. It regulates major histocompatibility complex class I (MHC-I) gene expression by interacting with chromatin modifiers, linking it to antiviral responses and immune evasion mechanisms. TRIM26 also modulates key signaling pathways, including NF-κB and Wnt/β-catenin, through targeted ubiquitination of proteins like IKKβ and β-catenin, influencing cell proliferation and apoptosis.
In viral infections, TRIM26 exhibits both proviral and antiviral effects. For instance, it restricts hepatitis B virus replication by degrading the viral core protein but may enhance Zika virus infection by suppressing interferon signaling. In cancer, TRIM26 displays context-dependent roles: acting as a tumor suppressor in hepatocellular carcinoma by promoting p53 stabilization, yet functioning as an oncogene in colorectal and cervical cancers by activating pro-survival pathways. Dysregulation of TRIM26 expression correlates with clinical outcomes, implicating its potential as a therapeutic target or diagnostic biomarker. Despite progress, its tissue-specific regulatory mechanisms and dual roles in immunity and cancer remain incompletely understood, warranting further exploration.
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