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Recombinant Human AHI1 Protein

  • 中文名: 重组人(AHI1)蛋白
  • 别    名: AHI 1; AHI-1; Ahi1; AHI1_HUMAN; Contatins SH3 and WD40 domains
货号: PA2000-5405
Price: ¥询价
数量:
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产品详情

纯度>90%SDS-PAGE.
种属Human
靶点AHI1
Uniprot NoQ8N157
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间1-609aa
氨基酸序列MPTAESEAKVKTKVRFEELLKTHSDLMREKKKLKKKLVRSEENISPDTIRSNLHYMKETTSDDPDTIRSNLPHIKETTSDDVSAANTNNLKKSTRVTKNKLRNTQLATENPNGDASVEEDKQGKPNKKVIKTVPQLTTQDLKPETPENKVDSTHQKTHTKPQPGVDHQKSEKANEGREETDLEEDEELMQAYQCHVTEEMAKEIKRKIRKKLKEQLTYFPSDTLFHDDKLSSEKRKKKKEVPVFSKAETSTLTISGDTVEGEQKKESSVRSVSSDSHQDDEISSMEQSTEDSMQDDTKPKPKKTKKKTKAVADNNEDVDGDGVHEITSRDSPVYPKCLLDDDLVLGVYIHRTDRLKSDFMISHPMVKIHVVDEHTGQYVKKDDSGRPVSSYYEKENVDYILPIMTQPYDFKQLKSRLPEWEEQIVFNENFPYLLRGSDESPKVILFFEILDFLSVDEIKNNSEVQNQECGFRKIAWAFLKLLGANGNANINSKLRLQLYYPPTKPRSPLSVVEAFEWWSKCPRNHYPSTLYVTVRGLKVPDCIKPSYRSMMALQEEKGKPVHCERHHESSSVDTEPGLEESKEVIKWKRLPGQENEDVFVLISPTMEEY
分子量96.4 kDa
蛋白标签GST-tag at N-terminal
缓冲液冻干粉
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.


参考文献

以下是3篇关于重组人AHI1蛋白的代表性文献概览(文献信息及摘要为模拟示例,仅供参考):

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1. **文献名称**:**"Cloning and Functional Analysis of Recombinant Human AHI1 Protein in Neuronal Development"**

**作者**:Smith J, et al. (2018)

**摘要**:本研究成功在大肠杆菌表达系统中重组表达了人AHI1蛋白,并验证了其参与调控神经细胞纤毛形成及Wnt信号通路的活性,揭示了其在神经元迁移中的关键作用。

2. **文献名称**:**"Structural Characterization of AHI1 and Its Interaction with Rab8A in Ciliogenesis"**

**作者**:Chen L, et al. (2020)

**摘要**:通过昆虫细胞表达系统制备了高纯度重组AHI1蛋白,结合质谱分析发现其WD40结构域与Rab8A直接互作,为解释AHI1缺陷导致Joubert综合征的分子机制提供了结构基础。

3. **文献名称**:**"AHI1 Knockout Disrupts Hippo Signaling via Recombinant Protein Rescue Experiments"**

**作者**:Wang Y, et al. (2021)

**摘要**:在HEK293细胞中过表达重组AHI1蛋白,证实其通过稳定YAP蛋白调控Hippo信号通路,并挽救了因AHI1缺失导致的细胞增殖异常,提示其在组织稳态中的新功能。

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**说明**:以上文献及内容为模拟创作,实际文献需通过PubMed、Google Scholar等平台检索关键词如“recombinant AHI1”“AHI1 protein function”获取。真实研究中,AHI1蛋白常与纤毛发生、神经发育疾病(如Joubert综合征)关联,其重组表达多用于互作组或信号通路研究。


背景信息

**Background of Recombinant Human AHI1 Protein**

The **AHI1** (Abelson helper integration site 1) protein, also known as Jouberin, is encoded by the *AHI1* gene located on human chromosome 6q23. It plays a critical role in neurodevelopment, particularly in cerebellar and retinal formation, and is implicated in cellular processes such as ciliogenesis, cell migration, and signaling. AHI1 contains multiple functional domains, including SH3 and WD40 repeats, which facilitate protein-protein interactions and participation in molecular pathways.

Mutations in the *AHI1* gene are associated with **Joubert syndrome**, a rare autosomal recessive disorder characterized by brain malformations, intellectual disability, and retinal degeneration. Studying AHI1 helps unravel mechanisms underlying neurodevelopmental disorders and ciliopathies.

Recombinant human AHI1 protein is produced via molecular cloning techniques, often using expression systems like *E. coli*, insect cells, or mammalian cells to ensure proper folding and post-translational modifications. It is purified via affinity chromatography, followed by rigorous quality assessments. This recombinant protein serves as a vital tool for *in vitro* and *in vivo* studies, aiding in deciphering AHI1’s structural-functional relationships, its role in disease pathology, and potential therapeutic targeting. Researchers also utilize it to screen molecules that modulate AHI1 activity, advancing drug discovery for related disorders.

In summary, recombinant AHI1 protein is essential for understanding neurodevelopmental biology and developing diagnostics or treatments for AHI1-linked diseases.

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