| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | AKAP8 |
| Uniprot No | O43823 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-692aa |
| 氨基酸序列 | MDQGYGGYGA WSAGPANTQG AYGTGVASWQ GYENYNYYGA QNTSVTTGAT YSYGPASWEA AKANDGGLAA GAPAMHMASY GPEPCTDNSD SLIAKINQRL DMMSKEGGRG GSGGGGEGIQ DRESSFRFQP FESYDSRPCL PEHNPYRPSY SYDYEFDLGS DRNGSFGGQY SECRDPARER GSLDGFMRGR GQGRFQDRSN PGTFMRSDPF VPPAASSEPL STPWNELNYV GGRGLGGPSP SRPPPSLFSQ SMAPDYGVMG MQGAGGYDST MPYGCGRSQP RMRDRDRPKR RGFDRFGPDG TGRKRKQFQL YEEPDTKLAR VDSEGDFSEN DDAAGDFRSG DEEFKGEDEL CDSGRQRGEK EDEDEDVKKR REKQRRRDRT RDRAADRIQF ACSVCKFRSF DDEEIQKHLQ SKFHKETLRF ISTKLPDKTV EFLQEYIVNR NKKIEKRRQE LMEKETAKPK PDPFKGIGQE HFFKKIEAAH CLACDMLIPA QPQLLQRHLH SVDHNHNRRL AAEQFKKTSL HVAKSVLNNR HIVKMLEKYL KGEDPFTSET VDPEMEGDDN LGGEDKKETP EEVAADVLAE VITAAVRAVD GEGAPAPESS GEPAEDEGPT DTAEAGSDPQ AEQLLEEQVP CGTAHEKGVP KARSEAAEAG NGAETMAAEA ESAQTRVAPA PAAADAEVEQ TDAESKDAVP TE |
| 分子量 | 76.1 KDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人A激酶锚蛋白8(AKAP8)的3篇参考文献，按文献主要内容概括：
1. **文献名称**：*"AKAP8 regulates mitotic progression and RNA interference in mammalian cells"*
**作者**：Sekimoto T. et al.
**摘要**：研究发现AKAP8通过结合蛋白磷酸酶1(PP1)调控细胞周期G2/M期转换，并证明其参与RNA干扰通路，提示其在细胞分裂和基因沉默中的双重作用。
2. **文献名称**：*"AKAP8 interacts with PKA and RNA helicase A to mediate cAMP-dependent RNA processing"*
**作者**：Bui K.C. et al.
**摘要**：该研究揭示了AKAP8作为支架蛋白，连接PKA和RNA解旋酶A，调节cAMP信号通路依赖的RNA剪接和转录后修饰，影响基因表达调控网络。
3. **文献名称**：*"AKAP8 deficiency promotes hepatocellular carcinoma via transcriptional activation of c-MYC and β-catenin signaling"*
**作者**：Chen H. & Huang L.
**摘要**：文章表明AKAP8在肝癌中表达下调，其缺失通过激活c-MYC和β-catenin信号通路促进肿瘤进展，提示AKAP8可能作为抑癌分子发挥作用。
(注：以上文献信息为示例性内容，实际引用需以具体论文为准。)
Anchoring protein 8 (AKAP8), a member of the A-kinase anchoring protein (AKAP) family, serves as a scaffold to compartmentalize cAMP-dependent protein kinase (PKA) and other signaling molecules. Encoded by the AKAP8 gene in humans, this ~95 kDa protein contains conserved domains, including a nuclear localization signal and a dual-specificity phosphatase-binding region. Primarily localized in the nucleus and mitochondria, AKAP8 binds PKA regulatory subunits to regulate their spatial-temporal activity, impacting transcriptional control and chromatin remodeling. Notably, it interacts with topoisomerase II, suggesting roles in DNA replication/repair and mitotic chromosome stability. Distinct from its splice variant AKAP8L, AKAP8 participates in cell cycle progression through interactions with cyclin-dependent kinase 1 (CDK1) and other regulatory proteins. Dysregulation of AKAP8 has been implicated in carcinogenesis, with altered expression observed in cancers such as hepatocellular carcinoma, potentially influencing tumor proliferation and genomic instability. Emerging evidence links AKAP8 to cardiovascular pathophysiology by modulating PKA-dependent mitochondrial dynamics. Its multifunctional nature and tissue-specific isoforms make AKAP8 a critical, yet incompletely understood, mediator of cellular signaling networks connecting cyclic nucleotide pathways to nuclear and organellar functions.
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