| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | AK3L1 |
| Uniprot No | Q9UIJ7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-227aa |
| 氨基酸序列 | MGASARLLRAVIMGAPGSGKGTVSSRITTHFELKHLSSGDLLRDNMLRGTEIGVLAKAFIDQGKLIPDDVMTRLALHELKNLTQYSWLLDGFPRTLPQAEALDRAYQIDTVINLNVPFEVIKQRLTARWIHPASGRVYNIEFNPPKTVGIDDLTGEPLIQREDDKPETVIKRLKAYEDQTKPVLEYYQKKGVLETFSGTETNKIWPYVYAFLQTKVPQRSQKASVTP |
| 分子量 | 50.27 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人AK3L1蛋白的3篇代表性文献摘要(基于近年研究整理，具体文献需进一步确认)：
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1. **文献名称**: *"Recombinant human adenylate kinase 3-like 1 (AK3L1) expression, purification, and enzymatic characterization"*
**作者**: Li X, et al.
**摘要**: 本研究报道了人AK3L1基因的克隆与大肠杆菌重组表达，通过镍柱亲和层析纯化获得高纯度蛋白。酶活分析表明AK3L1对ATP/GTP转换具有底物选择性，并依赖Mg²⁺激活，提示其在线粒体核苷酸代谢中的潜在功能。
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2. **文献名称**: *"AK3L1 modulates mitochondrial apoptosis via AMPK signaling in hepatocellular carcinoma cells"*
**作者**: Zhang Y, et al.
**摘要**: 文章发现AK3L1在肝癌细胞中通过调控AMPK/mTOR信号通路影响线粒体依赖性凋亡。重组AK3L1蛋白的过表达可诱导细胞周期阻滞，为靶向癌症代谢提供新思路。
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3. **文献名称**: *"Structural insights into the catalytic mechanism of AK3L1 by X-ray crystallography"*
**作者**: Wang H, et al.
**摘要**: 通过X射线晶体学解析AK3L1蛋白的三维结构，揭示了其活性位点的关键氨基酸残基(如Lys76、Asp135)，并阐明该蛋白催化腺苷酸转移的分子机制，为设计AK3L1抑制剂奠定结构基础。
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**注意**：以上文献信息为模拟生成，实际研究中请通过**PubMed/Google Scholar**检索关键词(如"AK3L1 recombinant")获取准确文献。若需具体文章，可提供研究方向(如疾病、结构等)，进一步辅助筛选。
Recombinant human AK3L1 (adenylate kinase 3-like 1) protein, also known as AK3. is a key enzyme involved in cellular energy metabolism. It belongs to the adenylate kinase family, which catalyzes the reversible transfer of phosphate groups between nucleotides, maintaining nucleotide homeostasis. Specifically, AK3L1 localizes to the mitochondrial matrix and facilitates the conversion of AMP and GTP to ADP and GDP, playing a critical role in mitochondrial nucleotide recycling. This activity links it to oxidative phosphorylation and energy balance regulation.
AK3L1 dysfunction has been associated with mitochondrial disorders, developmental defects, and heart disease, underscoring its physiological importance. Structural studies reveal a conserved adenylate kinase fold with three domains: a nucleoside monophosphate-binding domain, a nucleoside triphosphate-binding domain, and a central CORE domain. Recombinant AK3L1 is typically produced in bacterial or mammalian expression systems, enabling research into its enzymatic mechanisms and interactions.
Current applications include studying its role in ATP synthesis, apoptosis modulation, and mitochondrial DNA maintenance. Its recombinant form also serves as a tool for investigating metabolic disorders and screening potential therapeutic compounds targeting energy metabolism pathways. Recent work explores its relationship with hypoxia adaptation and neurodegenerative diseases, suggesting broader implications in cellular stress responses.
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