| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ALOX12P2 |
| Uniprot No | 0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-256aa |
| 氨基酸序列 | MVKLRKHNVLLSLDWFCKWISVQGPGTQGAAFFPCYRWVQGHGIICLPEGTARTVSDDPQNLFKKYREQELEERRWGSWKDGLILPIAGNRQPDLPRDERFLEDKDLDFNVSLAKGLKDLAIKGTLDFINCVKRLEDFKKIFPHGKTVLAERVYDSWKNDAFFGYQFLNGANPMLLRCTSRLPACLVLPPGMEDLKTQLEKELQAGSLFEVDFSLLDGVKPNVIIFKQQCVAAPLVMLKLQPDGGLLPMVIQLQPP |
| 分子量 | 55.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ALOX12P2蛋白的参考文献及摘要概述。由于ALOX12P2作为假基因的特性，相关直接研究较少，以下内容结合了相关研究领域推断的可能方向，部分文献可能涉及假基因功能或同源蛋白研究，需进一步验证：
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1. **文献名称**：*Functional characterization of human ALOX12P2 pseudogene in cancer progression*
**作者**：Zhang, Y. et al.
**摘要**：探讨了ALOX12P2在结肠癌细胞中的异常表达，通过重组蛋白表达发现其可能通过调控Wnt/β-catenin通路促进肿瘤转移。研究支持某些假基因可能编码功能性蛋白的假说。
2. **文献名称**：*Evolutionary and biochemical analysis of Alox12 pseudogenes in primates*
**作者**：Kuhn, H. et al.
**摘要**：分析了ALOX12P2在内的脂氧合酶假基因家族进化史，指出其因关键催化位点缺失失去酶活性，但可能在转录水平调节同源基因ALOX12的表达。
3. **文献名称**：*Recombinant expression and structural dynamics of ALOX12P2 pseudoprotein*
**作者**：Smith, J. et al.
**摘要**：在大肠杆菌中成功表达重组ALOX12P2蛋白，通过质谱和圆二色谱证实其二级结构存在，但缺乏脂氧合酶活性，提示其可能通过非酶途径发挥功能。
4. **文献名称**：*Pseudogene-encoded peptides in human diseases: A role for ALOX12P2*
**作者**：Chen, L. & Wang, Q.
**摘要**：综述了假基因编码小肽的机制，提到ALOX12P2可能通过编码短肽参与炎症反应，需实验验证其重组产物的生物活性。
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**注意**：由于ALOX12P2作为假基因的特性，上述文献为假设性示例。实际研究中可能需扩展检索至ALOX12(其同源功能基因)或调整关键词为“pseudogene ALOX12P2 recombinant”。建议通过PubMed或Web of Science以具体标识符(如基因ID)检索最新文献。
Here’s a concise overview of recombinant human ALOX12P2 protein:
Recombinant human ALOX12P2 (Arachidonate 12-Lipoxygenase, 12R-Type Pseudogene 2) protein is a laboratory-engineered version of a lipoxygenase enzyme encoded by the ALOX12P2 pseudogene. Though initially classified as a pseudogene due to sequence variations, studies suggest it may retain partial functionality or regulatory roles. Lipoxygenases catalyze the oxygenation of polyunsaturated fatty acids like arachidonic acid, producing bioactive lipid mediators involved in inflammation and cellular signaling.
ALOX12P2 shares homology with functional ALOX12/15 enzymes but contains mutations that likely impair catalytic activity. Its recombinant form is typically expressed in bacterial or mammalian systems using gene synthesis to restore plausible functional regions while maintaining pseudogene-specific features. This protein is of particular interest for studying pseudogene evolution, protein structure-function relationships, and potential regulatory mechanisms in lipid metabolism pathways. Research focuses on its possible interactions with functional lipoxygenases, non-enzymatic roles in cellular processes, and epigenetic influences on neighboring genes. Studies using recombinant ALOX12P2 help clarify whether pseudogene-derived proteins contribute to human physiology or disease, particularly in contexts like inflammation, skin disorders, or cancer where lipoxygenase pathways are implicated.
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