| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | AMN1 |
| Uniprot No | Q8IY45 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-213aa |
| 氨基酸序列 | MQGQITDSNISEILHPEVQTLDLRSCDISDAALLHLSNCRKLKKLNLNASKGNRVSVTSEGIKAVASSCSYLHEASLKRCCNLTDEGVVALALNCQLLKIIDLGGCLSITDVSLHALGKNCPFLQCVDFSATQVSDSGVIALVSGPCAKKLEEIHMGHCVNLTDGAVEAVLTYCPQIRILLFHGCPLITDHSREVLEQLVGPNKLKQVTWTVY |
| 分子量 | 49.4b kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人蛋白AMN1同源物(AMN1)的3篇参考文献示例，基于假设性研究内容整理：
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1. **文献名称**：*AMN1 Homolog in Human Cells Regulates Mitotic Exit and Cytokinesis*
**作者**：Zhang et al.
**摘要**：研究发现人源AMN1与酵母同源物功能保守，通过调控有丝分裂退出网络(MEN)影响胞质分裂。重组AMN1蛋白体外实验表明其与磷酸酶复合物相互作用，提示其在细胞周期进程中的作用。
2. **文献名称**：*Structural Insights into Human AMN1 and Its Role in Tumor Suppression*
**作者**：Kumar et al.
**摘要**：通过重组表达纯化人AMN1蛋白，解析其晶体结构，发现其通过结合CDK1抑制异常细胞增殖。体内实验表明AMN1下调与多种癌症进展相关，具有潜在抑癌功能。
3. **文献名称**：*Functional Analysis of Recombinant Human AMN1 in Drosophila Models*
**作者**：Chen et al.
**摘要**：利用果蝇模型表达重组人AMN1.揭示其通过Wnt信号通路调控细胞极性，支持AMN1在发育和疾病中的进化保守性，为研究其机制提供新模型。
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**备注**：由于实际研究中AMN1在人类中的直接文献较少，以上内容基于假设及类似功能蛋白研究模式推测。建议进一步结合基因别名(如FAMN1)或功能关键词(如“胞质分裂调控”)扩大检索。
**Background of the AMN1 Homolog (Anti-Mitotic Network 1):**
AMN1 (Anti-Mitotic Network 1) is a conserved protein initially identified in *Saccharomyces cerevisiae* as a critical regulator of mitotic exit and cell separation. It functions as part of the "mitotic exit network" (MEN) and "agonist of mitotic exit network" (AMEN) pathways, ensuring proper coordination between chromosome segregation and cytokinesis. In yeast, AMN1 acts as an antagonist to the MEN pathway, delaying mitotic exit under stress conditions to maintain genomic stability.
The human homolog, AMN1. shares structural and functional similarities, though its role in mammalian cells is less fully characterized. Studies suggest involvement in cell cycle regulation, particularly in the G1/S transition and spindle assembly. AMN1 is thought to interact with components of the anaphase-promoting complex/cyclosome (APC/C), influencing ubiquitination-mediated proteolysis of cell cycle regulators.
Recombinant human AMN1 protein (produced via bacterial or eukaryotic expression systems) is utilized to study its biochemical properties, interactions, and regulatory mechanisms. Dysregulation of AMN1 homologs has been implicated in cancer, where aberrant cell cycle progression is a hallmark. Current research focuses on elucidating its potential as a therapeutic target or biomarker for diseases linked to mitotic errors. Structural analyses reveal conserved domains critical for binding partners, offering insights into its evolutionary role in cell division fidelity.
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