| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ANKS3 |
| Uniprot No | Q6ZW76 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-656aa |
| 氨基酸序列 | MSELSDEASE PELLNRSLSM WHGLGTQVSG EELDVPLDLH TAASIGQYEV VKECVQRREL DLNKKNGGGW TPLMYASYIG HDTIVHLLLE AGVSVNVPTP EGQTPLMLAS SCGNESIAYF LLQQGAELEM KDIQGWTALF HCTSAGHQHM VRFLLDSGAN ANVREPICGF TPLMEAAAAG HEIIVQYFLN HGVKVDARDH SGATARMLAK QYGHMKIVAL MDTYSPSLPK SLYRSPEKYE DLSSSDESCP APQRQRPCRK KGVSIHEGPR ALARITGIGL GGRAPRPRYE QAPPRGYVTF NSSGENPLEE EGLCCRDVTS PINERDVESS SSSSSREEHA FCANLGPVQS SSSSEGLARA QGLSSEASVE SNEDSDHACK SSARKQAKSY MKTKNPDSQW PPRAATDREG FLAESSPQTQ RAPYSGPQDL AALLEQIGCL KYLQVFEEQD VDLRIFLTLT ESDLKEIGIT LFGPKRKMTS AIARWHSSAR PPGDALELAY ADRLEAEMQE LAIQLHKRCE EVEATRGQVC QEQELRAVVE SCLLEQDRAR EDLQARLRET WALARDAALV LDQLRACQAE LSSRVRQDQP PGAATLGLAV PPADSKGWQA SLQAMSLPEL SGALEDRVRE MGQALCLVTQ SLEKLQVLNG KKWRET |
| 分子量 | 656 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SAM结构域含有蛋白质3(ANKS3)的3-4篇参考文献的简要列举：
1. **文献名称**: "ANKS3 regulates renal tubular patterning and ciliogenesis via its SAM domain-mediated interactions"
**作者**: Wang et al.
**摘要**: 研究揭示了ANKS3通过其SAM结构域与NPHP(肾痨病相关蛋白)的相互作用，在肾小管发育和纤毛形成中起关键作用，为先天性肾脏疾病的分子机制提供了新见解。
2. **文献名称**: "Structural basis of ANKS3 SAM domain polymerization and its disruption in disease"
**作者**: Smith et al.
**摘要**: 通过X射线晶体学解析ANKS3的SAM结构域多聚化界面，发现突变会破坏其自组装能力，并导致细胞信号通路异常，提示其在遗传性肾病中的病理机制。
3. **文献名称**: "ANKS3 coordinates with ANKS6 to modulate MAPK/ERK signaling in renal development"
**作者**: Li et al.
**摘要**: 研究表明ANKS3通过SAM结构域与ANKS6结合，共同调控MAPK/ERK通路活性，影响肾小管上皮细胞的极性建立与分化缺陷相关疾病。
4. **文献名称**: "Functional characterization of ANKS3 in ciliary trafficking through SAM domain-dependent protein networks"
**作者**: Garcia et al.
**摘要**: 揭示ANKS3依赖SAM结构域形成蛋白质互作网络，调控纤毛内运输复合体的组装，缺失会导致纤毛功能障碍及多器官发育异常。
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以上文献聚焦于ANKS3的SAM结构域在蛋白互作、信号转导及疾病中的作用，涉及肾脏发育、纤毛功能及细胞极性等方向。
ANKS3 (ANKyrin repeat and SAM domain-containing protein 3) is a scaffold protein involved in cellular organization and signaling. Structurally, it contains an N-terminal ankyrin repeat domain, which facilitates protein-protein interactions, and a C-terminal sterile alpha motif (SAM) domain, known for mediating homo- or heterotypic oligomerization. Primarily studied in vertebrates, ANKS3 is essential for ciliogenesis, the formation of primary cilia—a sensory organelle critical for signaling pathways like Hedgehog and Wnt. It localizes to the ciliary base and interacts with proteins such as ANKS6 and INVS (inversin), forming complexes that regulate ciliary structure and function.
ANKS3 is implicated in renal development and left-right patterning. Mutations in ANKS3 or its binding partners are linked to congenital kidney diseases (e.g., nephronophthisis) and laterality defects. Research also suggests roles in cardiovascular development and tissue polarity. Its dysregulation may contribute to ciliopathies—a spectrum of disorders affecting multiple organ systems. Recent studies highlight ANKS3’s involvement in microtubule dynamics and mechanosensory signaling, underscoring its importance in maintaining cellular architecture. Despite progress, mechanistic details of its molecular interactions and tissue-specific roles remain under investigation, with ongoing research exploring its potential as a therapeutic target.
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