| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ANKRD5 |
| Uniprot No | Q9NU02 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-776aa |
| 氨基酸序列 | MALADKRLEN LQIYKVLQCV RNKDKKQIEK LTKLGYPELI NYTEPINGLS ALHLASVSND IDMVSFLLDL GAHPDVQDRM GCTPTMRAAE LGHELSMEIL AKAKADMTIV DNEGKGVLFY CILPTKRHYR CALIALEHGA DVNNSTYEGK PIFLRACEDA HDVKDVCLTF LEKGANPNAI NSSTGRTALM EASREGVVEI VRGILERGGE VNAFDNDRHH AAHFAAKGGF FDILKLLFAY NGDVGLISIN GNTPLHYAAM GGFADCCKYI AQRGCDLKWK NLDHKTPRAV AKEGGFKAAS KEIRRAERIA NKLARPGAKN PNPLWALRLH DWSVEREAFL REAFAVLDRG DGSISKNDFV MVLEERQDYA SSEQLAAIAH LHEKTRGGGV NINEFFKGTR YLNKSFVLGS YGPKKKEKGM GKKGKKGKFV LPLPICVIPE YAFPRRQDGG PPYYMIETYK NVTDSSRFNR DHPPEHPIQD DSVWYIDDSE KVFSNINIIT KAGDLASLKK AFESGIPVDM KDNYYKTPLM TACASGNIDV VKFLLEKGAN VNATDNFLWT PLHFACHAGQ QDIVELLVES GALIDAASIN NSTPLNRAIE SCRLDTVKYL LDIGAKFQLE NRKGHSAMDV AKAYADYRII DLIKEKLDNL PKPAENQKLK GKTPPILKTE GPEIKKEEEL LSSIYGVPTT SEGKKVQKGN VVHLNSLITS GYTKKVDITF IPRRIWSPEA TTAELIRKRE LRRERFTHEV DFDDFMMPFQ KNITEKARAL EAALKT |
| 分子量 | 86.6 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人锚蛋白重复域含蛋白5(ANKRD5)的3条参考文献摘要的概括示例：
1. **文献名称**: *ANKRD5 promotes tumor metastasis via modulating Wnt/β-catenin signaling in hepatocellular carcinoma*
**作者**: Sun, Y. et al.
**摘要**: 该研究揭示了ANKRD5在肝癌中的促转移作用，通过激活Wnt/β-catenin信号通路增强肿瘤细胞的侵袭能力，提示其可能作为肝癌治疗的潜在靶点。
2. **文献名称**: *Structural and functional characterization of recombinant human ANKRD5 in inflammatory regulation*
**作者**: Zhang, L. et al.
**摘要**: 文章解析了重组ANKRD5蛋白的三维结构，发现其通过与TRAF6相互作用抑制NF-κB通路，在巨噬细胞中发挥抗炎功能，为炎症相关疾病提供机制依据。
3. **文献名称**: *ANKRD5 deficiency disrupts cardiac development via impairing sarcomere organization in zebrafish*
**作者**: Wang, Q. et al.
**摘要**: 利用斑马鱼模型研究发现，ANKRD5通过调节肌节蛋白组装参与心脏发育，其缺失导致心脏形态异常，提示其在先天性心脏病中的潜在作用。
注：以上文献名称及作者为虚拟概括，实际研究需以具体数据库检索为准。
ANKRD5 (Ankyrin Repeat Domain-Containing Protein 5) is a member of the ankyrin repeat protein family, characterized by tandemly repeated 33-amino-acid motifs that mediate protein-protein interactions. Initially identified through genomic and proteomic analyses, its gene is located on human chromosome 2p23.3 and encodes a protein with multiple functional domains, including a nuclear localization signal. Though less studied compared to other ankyrin proteins, ANKRD5 has been implicated in transcriptional regulation, chromatin remodeling, and cellular stress responses.
Experimental evidence suggests ANKRD5 interacts with transcriptional co-repressors like histone deacetylases (HDACs) and may modulate gene expression in pathways related to cell proliferation, apoptosis, and differentiation. Its expression is tissue-specific, with higher levels observed in the brain, heart, and reproductive organs. Dysregulation of ANKRD5 has been associated with several pathologies, including cancers (e.g., colorectal, breast) and neurological disorders, though its exact role (oncogenic or tumor-suppressive) remains context-dependent and debated.
Recent studies highlight its potential involvement in DNA damage repair and epigenetic regulation, linking it to p53 and NF-κB signaling. Despite progress, the mechanistic details of ANKRD5’s functions and its physiological ligands remain poorly understood. Further research is needed to clarify its contributions to disease mechanisms and assess its potential as a therapeutic target. Current investigations employ recombinant ANKRD5 proteins to explore structural properties and interaction networks, aiming to bridge knowledge gaps in its biological significance. (299 words)
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