| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ANKRD50 |
| Uniprot No | Q9ULJ7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-743aa |
| 氨基酸序列 | MGHREIVEHLLDHGAEVNHEDVDGRTALSVAALCVPASKGHASVVSLLIDRGAEVDHCDKDGMTPLLVAAYEGHVDVVDLLLEGGADVDHTDNNGRTPLLAAASMGHASVVNTLLFWGAAVDSIDSEGRTVLSIASAQGNVEVVRTLLDRGLDENHRDDAGWTPLHMAAFEGHRLICEALIEQGARTNEIDNDGRIPFILASQEGHYDCVQILLENKSNIDQRGYDGRNALRVAALEGHRDIVELLFSHGADVNCKDADGRPTLYILALENQLTMAEYFLENGANVEASDAEGRTALHVSCWQGHMEMVQVLIAYHADVNAADNEKRSALQSAAWQGHVKVVQLLIEHGAVVDHTCNQGATALCIAAQEGHIDVVQVLLEHGADPNHADQFGRTAMRVAAKNGHSQIIKLLEKYGASSLNGCSPSPVHTMEQKPLQSLSSKVQSLTIKSNSSGSTGGGDMQPSLRGLPNGPTHAFSSPSESPDSTVDRQKSSLSNNSLKSSKNSSLRTTSSTATAQTVPIDSFHNLSFTEQIQQHSLPRSRSRQSIVSPSSTTQSLGQSHNSPSSEFEWSQVKPSLKSTKASKGGKSENSAKSGSAGKKAKQSNSSQPKVLEYEMTQFDRRGPIAKSGTAAPPKQMPAESQCKIMIPSAQQEIGRSQQQFLIHQQSGEQKKRNGIMTNPNYHLQSNQVFLGRVSVPRTMQDRGHQEVLEGYPSSETELSLKQALKLQIEGSDPSFNYKKETPL |
| 分子量 | 106.6 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4篇关于ANKRD50的虚拟参考文献示例(仅供参考，实际文献需通过学术数据库检索)：
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1. **文献名称**：*ANKRD50 regulates endosomal trafficking and cancer cell invasion through Rab GTPase interaction*
**作者**：Smith A, et al.
**摘要**：研究发现ANKRD50通过结合Rab家族GTP酶(如Rab11和Rab25)调控内体运输通路，影响肿瘤细胞的侵袭转移能力，提示其在癌症进展中的潜在作用。
2. **文献名称**：*Structural insights into the ankyrin repeat domain of ANKRD50 and its role in protein-protein interactions*
**作者**：Chen L, et al.
**摘要**：通过晶体结构解析ANKRD50的锚蛋白重复域，揭示其与DNA修复蛋白BRCA1的相互作用机制，表明其可能参与基因组稳定性调控。
3. **文献名称**：*ANKRD50 as a novel regulator of cytokinesis in mammalian cells*
**作者**：Wang Y, et al.
**摘要**：报道ANKRD50在细胞分裂末期(胞质分裂)中的作用，缺失ANKRD50会导致多核细胞形成，暗示其在细胞周期调控中的重要性。
4. **文献名称**：*Epigenetic silencing of ANKRD50 in colorectal cancer and its clinical implications*
**作者**：Kim H, et al.
**摘要**：发现结直肠癌中ANKRD50因启动子甲基化表达下调，其低表达与患者预后不良相关，提示其可能的肿瘤抑制功能。
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**说明**：以上为模拟示例，实际文献需通过PubMed、Google Scholar等平台检索真实研究。如需具体文献，请提供关键词或研究方向进一步筛选!
**Ankyrin Repeat Domain-Containing Protein 50 (ANKRD50)** is a member of the ankyrin repeat protein family, characterized by tandem repeats of ~33 amino acid motifs involved in mediating protein-protein interactions. Predominantly localized to endosomal and Golgi membranes, ANKRD50 plays a regulatory role in intracellular trafficking and membrane dynamics. Studies suggest its involvement in coordinating endosome-to-Golgi retrograde transport, modulating vesicle sorting and receptor recycling. Additionally, ANKRD50 interacts with components of the ESCRT (Endosomal Sorting Complex Required for Transport) machinery, influencing membrane remodeling processes and autophagy.
Research highlights its potential role in cellular stress responses and disease pathogenesis. Dysregulation of ANKRD50 has been linked to cancer progression, particularly in breast and colorectal cancers, where altered expression correlates with tumor invasiveness and metastasis. Its interaction with signaling pathways, such as TGF-β and NF-κB, implicates ANKRD50 in modulating inflammation and epithelial-mesenchymal transition (EMT). Despite these insights, the precise molecular mechanisms underlying its functions remain under investigation.
ANKRD50's structural versatility, conserved across species, underscores its biological significance. Further exploration of its interactions and regulatory networks may reveal therapeutic targets for cancer and trafficking-related disorders. Current studies focus on elucidating its roles in organelle homeostasis and disease, positioning ANKRD50 as a molecule of emerging interest in cell biology and translational research.
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