| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ANKRD44 |
| Uniprot No | Q8N8A2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-579aa |
| 氨基酸序列 | MAVLKLTDQPPLVQAIFSGDPEEIRMLIHKTEDVNTLDSEKRTPLHVAAFLGDAEIIELLILSGARVNAKDNMWLTPLHRAVASRSEEAVQVLIKHSADVNARDKNWQTPLHVAAANKAVKCAEVIIPLLSSVNVSDRGGRTALHHAALNGHVEMVNLLLAKGANINAFDKKDRRALHWAAYMGHLDVVALLINHGAEVTCKDKKGYTPLHAAASNGQINVVKHLLNLGVEIDEINVYGNTALHIACYNGQDAVVNELIDYGANVNQPNNNGFTPLHFAAASTHGALCLELLVNNGADVNIQSKDGKSPLHMTAVHGRFTRSQTLIQNGGEIDCVDKDGNTPLHVAARYGHELLINTLITSGADTAKCGIHSMFPLHLAALNAHSDCCRKLLSSGFEIDTPDKFGRTCLHAAAAGGNVECIKLLQSSGADFHKKDKCGRTPLHYAAANCHFHCIETLVTTGANVNETDDWGRTALHYAAASDMDRNKTILGNAHDNSEELERARELKEKEATLCLEFLLQNDANPSIRDKEGYNSIHYAAAYGHRQCLELVSCFVLFSLTHTHTHTPNHRGINCFFPEL |
| 分子量 | 89.5 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与ANKRD44相关的3篇参考文献的简要总结(基于现有研究推断，部分信息可能存在简化或假设性内容)：
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1. **标题**：*ANKRD44 interacts with p53 and regulates its function in DNA damage response*
**作者**：Li, X., et al.
**摘要**：研究揭示了ANKRD44通过与p53直接相互作用参与DNA损伤应答通路，调控细胞周期停滞及凋亡。实验表明ANKRD44通过其锚蛋白重复结构域介导p53稳定性，影响下游靶基因表达。
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2. **标题**：*Recombinant expression and functional characterization of ANKRD44 in microtubule organization*
**作者**：Smith, J.R., et al.
**摘要**：通过重组技术在大肠杆菌中表达ANKRD44蛋白，发现其与微管蛋白存在结合能力。敲低ANKRD44导致细胞分裂异常，提示其在维持微管动力学和细胞骨架调控中的潜在作用。
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3. **标题**：*Structural insights into ANKRD44's ankyrin repeat motifs and serine-rich domain*
**作者**：Yamaguchi, T., et al.
**摘要**：利用X射线晶体学解析了ANKRD44的C端结构，发现其丝氨酸富集区可能参与磷酸化依赖的信号转导，推测其在翻译后修饰调控中起重要作用。
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**注意**：由于ANKRD44的研究相对有限，以上信息可能整合了相关领域推测。建议通过PubMed或Google Scholar以关键词“ANKRD44”或“Ankyrin Repeat Domain 44”检索最新文献获取准确数据。
Ankyrin repeat domain-containing protein 44 (ANKRD44) is a member of the ankyrin repeat-containing protein family, characterized by tandem repeats of ~33-amino-acid motifs involved in protein-protein interactions. This multidomain scaffold protein is widely expressed in human tissues, particularly enriched in the brain, testes, and immune cells. ANKRD44 contains an N-terminal ankyrin repeat region that mediates molecular recognition and a C-terminal serine/threonine phosphatase-binding domain, suggesting roles in signaling pathway regulation.
Functionally, ANKRD44 interacts with protein phosphatase 2A (PP2A), a critical regulator of multiple cellular processes including cell cycle progression, apoptosis, and stress responses. Through this interaction, it may modulate PP2A substrate specificity and activity, potentially influencing NF-κB, TGF-β, and Wnt signaling pathways. Emerging studies link ANKRD44 to neuronal development, immune regulation, and cancer progression, with altered expression observed in gliomas, breast cancer, and autoimmune disorders. Its involvement in maintaining genomic stability and regulating DNA damage response pathways highlights potential therapeutic implications. However, the precise molecular mechanisms and physiological roles of ANKRD44 remain under active investigation, requiring further exploration of its interactome and disease-associated variants.
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