| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | APOB48R |
| Uniprot No | Q0VD83 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 71-170aa |
| 氨基酸序列 | RGSQNEGAGRLRGPGDDRRHEVGSSAVEQTWGWGDGSSHGSQAERQDSGAGETAKAARCQEPSAHLEARKKSKAGSGACQDRSGQAQERQESHEQEVNRE |
| 分子量 | 36.74 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人APOB48R蛋白的参考文献示例，涵盖其克隆、表达及功能研究：
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1. **文献名称**: *Molecular Cloning and Functional Characterization of the Human ApoB-48 Receptor*
**作者**: Hussain MM, et al.
**摘要**: 该研究成功克隆并表达了人APOB48R蛋白，证实其在肝脏中特异性介导乳糜微粒残体的摄取。通过体外实验，揭示了其与载脂蛋白B48(ApoB-48)的结合能力及与LDL受体的功能差异。
2. **文献名称**: *Recombinant Expression of ApoB48 Receptor in Mammalian Cells and Its Role in Lipid Homeostasis*
**作者**: Fisher EA, et al.
**摘要**: 利用哺乳动物细胞系统表达重组APOB48R蛋白，发现其通过调控低密度脂蛋白的代谢参与胆固醇稳态，并证实其与ApoB-48的特异性相互作用对动脉粥样硬化具有潜在影响。
3. **文献名称**: *Structural Insights into ApoB48 Recognition by Its Receptor via Recombinant Protein Complex Analysis*
**作者**: Young SG, et al.
**摘要**: 通过重组蛋白技术解析APOB48R的配体结合结构域，结合X射线晶体学揭示了其与ApoB-48的关键结合位点，为靶向药物开发提供了结构基础。
4. **文献名称**: *ApoB48 Receptor-Mediated Chylomicron Clearance: Functional Studies Using Recombinant Receptor Constructs*
**作者**: Krieger M, et al.
**摘要**: 通过构建重组APOB48R蛋白并模拟乳糜微粒代谢，验证了该受体在肝细胞中的内吞功能及其与脂蛋白颗粒结合的动力学特性。
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**注意**：以上文献为示例性质，实际引用需以真实发表的论文为准，建议通过PubMed或Google Scholar以关键词“ApoB48R recombinant”“APOB48 receptor function”等进一步检索。
**Background of Recombinant Human APOB48R Protein**
Apolipoprotein B48 receptor (APOB48R), also known as apolipoprotein B receptor, is a key protein involved in lipid metabolism and lipoprotein processing. APOB48R specifically binds to apolipoprotein B48 (apoB48), a truncated form of apoB100 essential for chylomicron assembly in the intestine. This receptor-ligand interaction facilitates the clearance of dietary lipid-rich particles, regulating postprandial lipid transport and maintaining systemic lipid homeostasis.
Structurally, APOB48R shares homology with other lipoprotein receptors, featuring ligand-binding domains that mediate cellular uptake of apoB48-containing lipoproteins. Dysregulation of APOB48R has been implicated in metabolic disorders, including hyperlipidemia, atherosclerosis, and insulin resistance, highlighting its physiological and pathological relevance.
Recombinant human APOB48R protein is engineered using heterologous expression systems (e.g., mammalian or bacterial cells) to produce functional, high-purity protein for research. It serves as a critical tool for studying receptor-ligand interactions, lipid metabolism pathways, and drug screening for cardiovascular or metabolic diseases. Recent studies also explore its therapeutic potential in modulating dyslipidemia.
Overall, APOB48R remains a focal point in understanding lipoprotein biology and developing targeted interventions for lipid-related disorders.
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