| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | APG3L |
| Uniprot No | Q9NT62 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-314aa |
| 氨基酸序列 | MQNVINTVKGKALEVAEYLTPVLKESKFKETGVITPEEFVAAGDHLVHHCPTWQWATGEELKVKAYLPTGKQFLVTKNVPCYKRCKQMEYSDELEAIIEEDDGDGGWVDTYHNTGITGITEAVKEITLENKDNIRLQDCSALCEEEEDEDEGEAADMEEYEESGLLETDEATLDTRKIVEACKAKTDAGGEDAILQTRTYDLYITYDKYYQTPRLWLFGYDEQRQPLTVEHMYEDISQDHVKKTVTIENHPHLPPPPMCSVHPCRHAEVMKKIIETVAEGGGELGVHMYLLIFLKFVQAVIPTIEYDYTRHFTM |
| 分子量 | 51.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人泛素样连接酶APG3L(ATG3)的3篇参考文献及其摘要概括：
1. **文献名称**：*Mammalian autophagy: molecular mechanisms and physiological functions*
**作者**：Mizushima N., Yoshimori T., Ohsumi Y.
**摘要**：综述中提出APG3L(ATG3)作为自噬关键蛋白，通过LC3脂化过程参与自噬体形成，强调其泛素样连接酶活性对细胞稳态的重要性。
2. **文献名称**：*The Atg12-Atg5 conjugate has a novel E3-like activity for protein lipidation in autophagy*
**作者**：Hanada T., Ohsumi Y.
**摘要**：研究揭示APG3L与Atg12-Atg5复合物协同作用，促进LC3(哺乳动物同源物为ATG8)的泛素样修饰，为自噬体膜延伸提供分子基础。
3. **文献名称**：*Structure of the human ATG3–LC3B complex reveals the molecular mechanism of LC3 lipidation in autophagy*
**作者**：Sou Y.S., et al.
**摘要**：通过晶体结构分析，阐明APG3L催化LC3B与磷脂酰乙醇胺结合的分子机制，揭示了其特异性识别底物及泛素样修饰的关键结构域。
*注*：APG3L即ATG3.主要参与自噬过程的泛素样反应。若需实验类文献，建议补充具体研究主题(如疾病关联或调控机制)。
Recombinant human ubiquitin-like conjugating enzyme APG3L (Autophagy-related protein 3 homolog), commonly referred to as ATG3. is a key component of the autophagy machinery, a cellular degradation pathway critical for maintaining homeostasis. APG3L functions as an E2-like enzyme in the ubiquitin-like conjugation system, facilitating the covalent attachment of the autophagy-related protein LC3 (microtubule-associated protein 1A/1B-light chain 3) to phosphatidylethanolamine (PE) on autophagosomal membranes. This lipidated LC3-PE conjugate (LC3-II) is essential for autophagosome formation, cargo recruitment, and membrane expansion during autophagy. APG3L interacts with the E1-activating enzyme ATG7 and the E3-like complex ATG12-ATG5-ATG16L1 to mediate LC3 lipidation. Structurally, APG3L contains an N-terminal membrane-binding domain and a C-terminal catalytic core. Dysregulation of APG3L has been implicated in neurodegenerative disorders, cancer, and microbial infection due to its role in autophagy-dependent clearance of protein aggregates, damaged organelles, and pathogens. Recombinant APG3L, produced via heterologous expression systems (e.g., E. coli or mammalian cells), enables biochemical studies of autophagy mechanisms, drug screening for autophagy modulators, and therapeutic exploration targeting autophagy-related diseases. Its recombinant form retains enzymatic activity and structural integrity, making it valuable for in vitro reconstitution assays and structure-function analyses.
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